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Conjugal transfer of erm(B) and multiple tet genes from Lactobacillus spp. to bacterial pathogens in animal gut, in vitro and during food fermentation.

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Relation http://ir.cftri.com/13913/
https://doi.org/10.1016/j.foodres.2018.09.046
 
Title Conjugal transfer of erm(B) and multiple tet genes from Lactobacillus spp. to
bacterial pathogens in animal gut, in vitro and during food fermentation.
 
Creator Surya Chandra Rao, Thumu
Prakash, M. Halami
 
Subject 02 Bacteriology
04 Fermentation Technology
 
Description Three strains of Lactobacillus comprising Lactobacillus salivarius (CHS-1E and CH7-1E) and Lactobacillus reuteri
(CH2-2) previously isolated from chicken meat were analyzed for their transferability of antibiotic resistance
(AR) genes to pathogenic strains under in vivo, in vitro, and during food fermentation. For in vivo model, Albino
Wistar rats were inoculated with 1010 CFU/g/ml of Enterococcus faecalis JH2-2 (recipient). After 7 days, either of
two donors L. salivarius CH7-1E or L. reuteri [harbouring erythromycin and tetracycline resistance genes] were
introduced at a concentration of 109 CFU/ml daily for 1 week. Two days after donor introduction, there was a
stable increase in the number of transconjugants in the animal faeces from 102 to 103 CFU/g and presented erm
(B), tet(M), tet(L) and tet(W) in their genome like donor strains. Similar observations were made with in vitro
filter mating between CHS-1E, CH2-2 and CH7-1E and E. faecalis JH2-2 with transfer frequencies of 1×10−4,
3.8×10−3 and 2×10−3 per donor cell respectively. With the results obtained in vivo and in vitro, the AR
transferability of donor strains was estimated during food fermentation (chicken sausage, fermented milk or idli
batter) with pathogenic recipient strains added as contaminants. At the end of mating period, phenotypic resistance
to erythromycin and tetracycline in Listeria monocytogenes and Yersinia enterocolitica strains was observed.
This study showed the ability of food borne Lactobacillus in diffusing their AR traits in diverse natural
environments increasing their concern of AR dissemination in the food chain when used as food additives and/or
probiotics.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13913/1/Food%20Research%20International%20116%20%282019%29%201066%E2%80%931075.pdf
Surya Chandra Rao, Thumu and Prakash, M. Halami (2019) Conjugal transfer of erm(B) and multiple tet genes from Lactobacillus spp. to bacterial pathogens in animal gut, in vitro and during food fermentation. Food Research International, 116. pp. 1066-1075. ISSN 0963-9969