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In vivo antidiabetic and antioxidant potential of Psychotria dalzellii in streptozotocin-induced diabetic rats.

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Relation http://ir.cftri.com/13914/
https://doi.org/10.1016/j.sajb.2018.12.006
 
Title In vivo antidiabetic and antioxidant potential of Psychotria dalzellii in
streptozotocin-induced diabetic rats.
 
Creator Abhishek, M.
Somashekaraiah, B. V.
Shylaja, M. Dharmesh
 
Subject 04 Diabetes Mellitus
04 Medicinal Plants
 
Description Psychotria species have been known forwide array of health benefits as per traditional knowledge and only a few
scientific evidences are available. In the current study in vivo antidiabetic property of methanol extract of
Psychotria dalzellii (MEPD) was elucidated in alloxan-induced type 1 diabetes in Wistar rats. MEPD, acetone
and ethylacetate extractswere examined for potential anti-diabetic properties (α-amylase and α-glucosidase inhibition)
in in vitro and the significantly potent extract – MEPD was analyzed for in vivo antioxidant activity.
Blood glucose levels, TBARS, antioxidant, creatinine, alkaline phosphatase etc., were measured. Results of the
study revealed that, oral intubation of 400 mg kg−1b.w. of MEPD for a period of 20 days prior inducing diabetes
resulted in the normalization of blood glucose level (259.83±10.11 mg/dL as opposed to induced 389.5±8.97;
healthy animals had 129.16 ± 4.12 mg/dL glucose) suggesting the antidiabetic potency of MEPD. Biochemical
analysis substantiated the antidiabetic property of MEPD as revealed by normalization of altered TBARS/oxidant
and antioxidant levels. Results were further substantiated by in vitro studies, where MEPD exhibited good antioxidant
activity – reducing power activity (IC50–8.81 U/mg vs Ascorbic acid – 6.21 U/mg;MEPD); hydrogen peroxide
activity (IC50−26.57 μg/mL vs Ascorbic acid 19.12 μg/mL); MEPD inhibited α-amylase and α-glucosidase
activity with (IC50 47.11±2.8 μg/mL vs Acarbose – IC50 40.86±1.7 μg/mL); (IC50 40.34±1.2 μg/mL vs Acarbose
– IC50 40.99±2.3 μg/mL); providing evidence for the first time for the antidiabetic property of P. dalzellii. The potent
anti-type I diabetic property could be due to antioxidant and inhibition of α-glucosidase/amylase which results
in control of release of glucose levels into the circulation. Phytochemical analysis of revealed that MEPD is
enriched in phenolics and flavonoids compound. In order to understand its probable mechanism of action, antioxidant
status during healthy, diabetic and sample treated groups of animals were also investigated.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13914/1/South%20African%20Journal%20of%20Botany%20121%20%282019%29%20494%E2%80%93499.pdf
Abhishek, M. and Somashekaraiah, B. V. and Shylaja, M. Dharmesh (2019) In vivo antidiabetic and antioxidant potential of Psychotria dalzellii in streptozotocin-induced diabetic rats. South African Journal of Botany, 121. pp. 494-499.