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Sublichenin, a new subtilin-like lantibiotics of probiotic bacterium Bacillus licheniformis MCC 2512T with antibacterial activity.

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Relation http://ir.cftri.com/13916/
https://doi.org/10.1016/j.micpath.2018.12.044
 
Title Sublichenin, a new subtilin-like lantibiotics of probiotic bacterium Bacillus
licheniformis MCC 2512T with antibacterial activity.
 
Creator Prakash, M. Halami
 
Subject 11 Antibiotics Chemistry
02 Bacteriology
 
Description Probiotic bacteria with antibacterial activity is of desirable trait since they can check the growth of pathogenic
bacteria besides exhibiting health benefits to host. Aim of this study was to characterize Bacillus licheniformis
MCC 2512T (MCC 2512), a potential probiotic culture for its ability to produce subtilin-like antibiotics. The antimicrobial
compound produced by MCC 2512 was identified and characterized using subtilin-specific cell reporter,
Bacillus subtilis 168:BS2 (BS2). Induction of β-gal by the test culture suggested the ability of B. licheniformis
to produce subtilin-like lantibiotic. Subsequently, DNA sequencing of major lanS-operon was carried out,
wherein sequencing results showed that lan cluster of MCC 2512 resembles entianin (etn) type. Upon lan-S
disruption, the bacterial culture lost antimicrobial activity as well as ability to induce β-gal with BS2 reporter.
High amount of succinylated form of antibiotics produced by wild type and un-succinylated form by engineered
strain of B. subtilis 15029p clearly indicates that MCC 2512 is indeed an inter-specific subtilin-like (named as
sublichenin) lantibiotic producer. Partially concentrated sublichenin preparation exhibited strong antibacterial
activity against food-borne pathogens and antibiotic resistant (AR) lactic acid bacteria (LAB) with a minimum
inhibitory concentration in the range of 6–10 and 0.5–1.5 μg/ml, respectively. Production of lantibiotic, sublichenin
by a probiotic bacterium of B. licheniformis MCC 2512T and its antibacterial activity against food associated
AR LAB is a new information reported in this study.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13916/1/Microbial%20Pathogenesis%20128%20%282019%29%20139%E2%80%93146.pdf
Prakash, M. Halami (2019) Sublichenin, a new subtilin-like lantibiotics of probiotic bacterium Bacillus licheniformis MCC 2512T with antibacterial activity. Microbial Pathogenesis, 128. pp. 139-146.