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Dataset on exogenous application of salicylic acid and methyl jasmonate and the accumulation of caffeine in young leaf tissues and catabolically inactive endosperms.

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Relation http://ir.cftri.com/13932/
http://dx.doi.org/10.1016/j.plgene.2015.09.002
 
Title Dataset on exogenous application of salicylic acid
and methyl jasmonate and the accumulation
of caffeine in young leaf tissues and catabolically
inactive endosperms.
 
Creator Avinash, Kumar
Gyanendra Kumar, Naik
Giridhar, P.
 
Subject 04 Coffee
 
Description Exogenous exposure of coffee plants to 50 μM and 500 μM salicylic acid through liquid hydroponic medium or the exposure to volatile fumes of methyljasmonate was carried out to study the role of salicylic acid and methyljasmonate on the accumulation of caffeine and other methylxanthines like 7-methylxanthine, theobromine and theophylline. Transcript levels of the first, second and third N-methyltransferase involved in the core caffeine biosynthetic pathway namely, xanthosine methyltransferase (XMT), methylxanthine methyltransferase (MXMT) and di-methylxanthine methyltransferase (DXMT) was investigated by semi-quantitative RT-PCR for validating the reason behind the changes of caffeine biosynthetic potential under the influence of the two analogues of plant phytohormones. Maturing coffee fruits are known to be biologically inactive with respect to caffeine biosynthetic activity in the endosperms. To understand this, fruits were treated with different doses of salicylic acid in a time-course manner and the de-repression of tissue maturation-mediated knockdown of caffeine biosynthesis by exogenously applied salicylic acid was achieved. In our companion paper [1] it was shown that the repression of NMT genes during the dry weight accumulation phase of maturing endosperm could be relaxed by the exogenous application of salicylic acid and methyljasmonate. A probable model based on the work carried out therein and based on other literature [2], [3], [4] was proposed to describe that the crosstalk between salicylic acid or methyljasmonate and the ABA/ethylene pathway and might involve transcription factors downstream to the signaling cascade.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13932/1/Data%20inBrief13%282017%2922%E2%80%9327.pdf
Avinash, Kumar and Gyanendra Kumar, Naik and Giridhar, P. (2017) Dataset on exogenous application of salicylic acid and methyl jasmonate and the accumulation of caffeine in young leaf tissues and catabolically inactive endosperms. Data in Brief, 13. pp. 22-27.