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Biochemical characterization of a key step involved in 2H4MB production in Decalepis hamiltonii.

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Relation http://ir.cftri.com/13945/
https://dx.doi.org/10.1016/j.jplph.2017.04.006
 
Title Biochemical characterization of a key step involved in
2H4MB production in Decalepis hamiltonii.
 
Creator Kiran, K.
Pradeep, M. G.
Priyanka, P. S.
Giridhar, P.
 
Subject 08 General Biochemistry
Swallow root
 
Description Decalepis hamiltonii is widely known for its flavour molecule 2-Hydroxy-4-
Methoxy Benzaldehyde (2H4MB), a structural isomer of vanillin. As the biosynthetic
pathway of 2H4MB is not known, we hypothesised 2H4MB origins could be from
phenylpropanoid pathway (PPP). Accordingly, a study was conducted using PPP inhibitors
(viz. piperonylic acid, MDCA and propanil) against in vitro root cultures of D. hamiltonii to
find the branch of PPP which catalyses the 2H4MB formation. HPLC analysis was carried
out to quantify 2H4MB levels in control and respective inhibitor treated root cultures in vitro.
The results obtained revealed that piperonylic acid did not inhibit 2H4MB biosynthesis in the
given period, whereas MDCA and propanil had the marked inhibitory effect. The inhibitory
effect was evident with 13.2, 33.6 and 37.9% decrease in 2H4MB levels at 50, 100 and 150
mM concentration of MDCA respectively in comparison with control roots. Similarly, the
inhibitory effect of propanil on 2H4MB biosynthesis was obvious with 23.7, 49.5 and 57.9%
decrease in 2H4MB levels at 50, 100 and 150 μM concentration of inhibitor respectively
when compared with control roots. Propanil showed a greater slow down effect on 2H4MB
biosynthesis compared to MDCA. Incorporation of 0.1, 0.5 and 1.0 mM ferulic acid as a
precursor to in vitro root cultures of D. hamiltonii showed an increase in 2H4MB levels at the
rate of 3.1, 107 and 94.1% respectively as quantified by HPLC analysis. However, ferulic
acid in conjunction with propanil did not show any increase in 2H4MB levels. This clearly
explains that ferulic acid is channelled through the 4-CL (4-coumarate CoA ligase) enzyme,
where it would be converted to feruloyl-CoA and could be further converted to 2H4MB in D.
hamiltonii.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13945/1/Journal%20of%20Plant%20Physiology%202017.pdf
Kiran, K. and Pradeep, M. G. and Priyanka, P. S. and Giridhar, P. (2017) Biochemical characterization of a key step involved in 2H4MB production in Decalepis hamiltonii. Journal of Plant Physiology, 214. pp. 74-80. ISSN 0176-1617