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Enhanced bioaccessibility of green tea polyphenols and lipophilic activity of EGCG octaacetate on gram-negative bacteria.

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Relation http://ir.cftri.com/13947/
https://doi.org/10.1016/j.lwt.2019.01.064
 
Title Enhanced bioaccessibility of green tea polyphenols and lipophilic activity of
EGCG octaacetate on gram-negative bacteria.
 
Creator Kalaiselvi, I.
Rajalakshmi, P.
Pushpa, S. Murthy
Nagarajan, S.
 
Subject 08 Tea
 
Description The polyphenol acetates derived from green tea (Camellia sinensis. L) catechins, effectively increases the
bioaccessibility as evaluated by simulated invitro enzymatic digestion method. The acetylated polyphenols
showed significantly higher bioaccessibility (60.13 ± 0.3%) with respect to their precursors (31.80 ± 0.7%).
The antibacterial activity of major catechins and their acetates on food-borne pathogens indicated the minimum
inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) respectively for gram-positive
bacteria (GPB)– Bacillus subtilis (EGCG, 130 μg/mL and 150 μg/mL; EGCG octaacetate 100 μg/mL and 120 μg/
mL), Staphylococcus aureus (EGCG, 200 μg/mL and 250 μg/mL; EGCG octaacetate 150 μg/mL and 200 μg/mL)
and the gram-negative bacteria (GNB)- Escherichia coli (EGCG, 580 μg/mL and 700 μg/mL; EGCG octaacetate
250 μg/mL and 300 μg/mL) and Yersinia enterocolitica (EGCG, 620 μg/mL and 740 μg/mL; EGCG octaacetate
280 μg/mL and 330 μg/mL). The prepared EGCG octaacetate had higher inhibitory effect against both GNB and
GPB, than EGCG, which showed moderate activity on GPB and less effect on GNB. This is attributed to its
lipophilic nature, a distinctive property, as evaluated by lipophilicity test. It is further substantiated with
membrane permeability assay using fluorescent microscopy and the morphological alterations on E. coli cells by
scanning electron microscopy (SEM) studies.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13947/1/LWT%20-%20Food%20Science%20and%20Technology%20105%20%282019%29%20103%E2%80%93109.pdf
Kalaiselvi, I. and Rajalakshmi, P. and Pushpa, S. Murthy and Nagarajan, S. (2019) Enhanced bioaccessibility of green tea polyphenols and lipophilic activity of EGCG octaacetate on gram-negative bacteria. LWT - Food Science and Technology, 105. pp. 103-109. ISSN 0023-6438