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Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/13925/
https://doi.org/10.1016/j.biortech.2019.02.044
 
Title Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum.
 
Creator Rochak, M.
Richa, Sharma
Raghavarao, K. S. M. S.
 
Subject 01 Algae
05 Processing and Engineering
 
Description Aqueous two-phase extraction (ATPE) of R-Phycoerythrin (R-PE), a fluorescent and commercially valuable protein, was carried out in two parallel schemes. In scheme-1, ATPE alone was employed, and in scheme-2, process-integration was attempted, where primary extract was subjected to precipitation before standardization of process parameters of ATPE. Scheme-1 with polyethylene glycol (PEG)-3350/potassium phosphate system has resulted in very low R-PE yield (26% w/w) without much enrichment in purity (0.3). In scheme-2, PEG-1450/potassium phosphate system (pH 6, tie-line length 12.26% and lower volume-ratio) has resulted in higher R-PE purity (0.74) with 72% yield. R-PE containing PEG-rich (top) phase was subjected to ultrafiltration, to remove phase forming components, resulting in further increase in R-PE purity (1.1). Overall enrichment in R-PE purity of 11-fold with 57% (w/w) yield and removal of about 95% of total sugars was achieved in scheme-2. Intactness of R-PE after processing was confirmed by absorbance and emission spectrum analysis.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13925/1/Bioresource%20Technology%20Volume%20280%2C%20May%202019%2C%20Pages%20277-286.pdf
Rochak, M. and Richa, Sharma and Raghavarao, K. S. M. S. (2019) Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum. Bioresource Technology, 280. pp. 277-286. ISSN 0960-8524