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In Vitro Comparative Analysis of Probiotic and Functional Attributes of Indigenous Isolates of Bifidobacteria.

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Relation http://ir.cftri.com/13950/
https://doi.org/10.1007/s00284-018-1615-9
 
Title In Vitro Comparative Analysis of Probiotic and Functional Attributes
of Indigenous Isolates of Bifidobacteria.
 
Creator Sajan, C. Achi
Prakash, M. Halami
 
Subject 02 Bacteriology
04 Functional foods
 
Description In the present study, probiotic, safety and functional characteristics of eight indigenous bifidobacterial isolates were compared
to identify suitable strains for functional food application. Among the isolates, six strains of Bifidobacterium longum and
one each of Bifidobacterium breve and Bifidobacterium bifidum were identified by 16S rRNA, xfp and hsp60 gene sequencing.
Diversity among these strains was established by RAPD and Rep-PCR. Genes associated with sortase-dependent pili
(SDP) (credited for role in adhesion) and serpin (immunomodulation) which can serve as potential marker genes for rapid
identification of probiotic Bifidobacterium, was also evaluated. All the isolates exhibited potential probiotic, functional (antimicrobial
activity, antioxidant activity, phytase activity, milk fermentation ability) and safety attributes. However, among
them, B. breve NCIM5671 exhibited, better tolerance to low pH, amylase activity and exopolysaccharide producing ability.
B. bifidum NCIM5697 and B. longum NCIM5672 demonstrated higher adherence ability to Caco-2 cells. NCIM5697 also
displayed exopolysaccharide producing ability while NCIM5672 showed strong antibacterial activity against pathogens tested.
Further, with respect to presence of adhesion marker genes, disparity was observed among B. longum strains. B. longum
NCIM5684 and B. longum NCIM5686 displayed presence of subunits of SDP reported to be present in B. breve. In addition,
B. longum NCIM5686 also lacked SDP present in all other B. longum isolates. B. breve NCIM5671, B. longum NCIM5672
and B. bifidum NCIM5697 with appreciable traits qualifies as potential probiotic cultures. Further, the variations observed
in molecular and functional characteristics of isolates signify genetic diversity among the cultures.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13950/1/Current%20Microbiology%20%282019%29%2076304%E2%80%93311.pdf
Sajan, C. Achi and Prakash, M. Halami (2019) In Vitro Comparative Analysis of Probiotic and Functional Attributes of Indigenous Isolates of Bifidobacteria. Current Microbiology, 76. pp. 304-311.