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Ascorbyl stearate stimulates cell death by oxidative stress-mediated apoptosis and autophagy in HeLa cervical cancer cell line in vitro.

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Relation http://ir.cftri.com/13953/
https://doi.org/10.1007/s13205-019-1628-5
 
Title Ascorbyl stearate stimulates cell death by oxidative stress-mediated
apoptosis and autophagy in HeLa cervical cancer cell line in vitro.
 
Creator Shirish, D. Mane
Akhilender Naidu, K.
 
Subject 09 Human Physiology
03 Citrus fruits
 
Description In this study, Asc-s was evaluated for anti-cancer effect using cervical cancer cells (HeLa). Results determine that Asc-s
treatment-induced dose-dependent inhibition of proliferation of HeLa cells and induced apoptosis. Flow-cytometry analysis
shows Asc-s treatment-induced accumulation of cells at sub-G0/G1 stage of cell cycle and induced apoptosis as confirmed
by DAPI, propodium iodide, and acridine staining in HeLa cells. Asc-s entered the cells and metabolized to ascorbate and
stearate moieties, increased membrane permeability, and decreased membrane fluidity in HeLa cells. Asc-s treatment-induced
dose-dependent increase in autophagy protein LC3-II, mRNA levels and decreased Nrf-2 levels in HeLa cells. It is hypothesized
that both ascorbyl radical and stearoyl moieties of Asc-s induced cytotoxicity by generating reactive oxygen species
(ROS) and modulating membrane fluidity/permeability leading to apoptosis/autophagy of HeLa cells. Thus, our findings
demonstrate that Asc-s as anti-proliferative and apoptosis inducing compound in cervical cancer cells.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13953/1/3%20Biotech%20%282019%29%209115.pdf
Shirish, D. Mane and Akhilender Naidu, K. (2019) Ascorbyl stearate stimulates cell death by oxidative stress-mediated apoptosis and autophagy in HeLa cervical cancer cell line in vitro. 3 Biotech, 9. pp. 1-15.