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Transcription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/14229/
https://doi.org/10.1016/j.fm.2019.103336
 
Title Transcription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1.
 
Creator Deepesh, Panwar
Mukesh, Kapoor
 
Subject 09 Food Microbiology
 
Description Plantderivedgalactomannooligosaccharides(GMOS)areanemergingclassofprebiotics,butnoinformationis available on theirutilization in lactobacilli atthe molecular level. Thecurrent studyaimed atidentifying the geneticlociinvolvedinthetransportandcatabolismoflocustbeangumderivedGMOSinLactobacillusplantarum WCFS1. Substrate depletion study showed that L. plantarum WCFS1 can metabolize only short chain GMOS (degreeofpolymerization;DP≤3).GlobaltranscriptomemicroarrayprofilingofL.plantarumWCFS1revealed differential expression when GMOS or control sugars (glucose, galactose, and mannose) were used as a sole carbohydratesource.Twogeneticlociinvolvedincellobiose(~3.2kb)andoligo-sucrose(~7.3kb)utilizationin L. plantarumWCFS1werehighlyup-regulatedupto8.3 andupto6.7-fold,respectivelyby GMOSutilization. qRT-PCR studies of the selected gene clusters showed correlation with microarray data. Altogether, transcriptomeandqRT-PCRstudiesofL.plantarumWCFS1suggestedthatun-substitutedmannobiose(DP2)mightbe metabolized by proteins encoded by the cellobiose operon while, substituted DP2 (galactomannose) and DP3 (galactomannobiose)weremostlikelytransportedandcatabolizedbytheoligo-sucroseutilizationlociencoded proteins.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14229/1/Food%20Microbiology%2086%20%282022%29%20103336.pdf
Deepesh, Panwar and Mukesh, Kapoor (2020) Transcription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiology, 86. pp. 1-8.