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OsPLB gene expressed during seed germination encodes a phospholipase in rice.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/14279/
https://doi.org/10.1007/s13205-019-2016-x
 
Title OsPLB gene expressed during seed germination encodes
a phospholipase in rice.
 
Creator Achintya Kumar, Dolui
Latha, M.
Vijayaraj, P.
 
Subject 05 Enzymes
01 Rice
 
Description Hydrolysis of phospholipid monolayer by phospholipases is an important event in the mobilization of stored lipids for seed
germination. However, the identification and functional characterization of cereal phospholipases, especially during rice
germination, are limited. In the present study, we have identified and characterized a phospholipase OsPLB gene expressed
during germination. The full-length coding region of OsPLB was cloned into pRSETA as well as pYES2/NTC vector. The
recombinant protein was successfully expressed in both E. coli and Saccharomyces cerevisiae. The recombinant protein
was purified to homogeneity by affinity chromatography, and it was further confirmed by MS/MS analysis. In vitro lipase
assay and lipidome analysis using high-resolution mass spectrometry showed phosphatidylcholine (PC) specific phospholipase
B activity. The results revealed that protein encoded by OsPLB gene prefers to hydrolyze PCs with C28, C32, and
C34 containing unsaturated fatty acids. Collectively, the present study describes the identification and characterization of a
phospholipase B, which hydrolyze PC, a major component of phospholipid monolayer covering storage lipid, as an initial
event during rice seed germination.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14279/1/3%20Biotech%20%282020%29%201030.pdf
Achintya Kumar, Dolui and Latha, M. and Vijayaraj, P. (2020) OsPLB gene expressed during seed germination encodes a phospholipase in rice. 3 Biotech, 10. pp. 1-9.