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Molecular and functional characterization of the Listeria monocytogenes RecA protein: insights into the homologous recombination process.

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Relation http://ir.cftri.com/14281/
https://doi.org/10.1016/j.biocel.2019.105642
 
Title Molecular and functional characterization of the Listeria monocytogenes
RecA protein: insights into the homologous recombination process.
 
Creator Debika, Ojha
Neelakanteshwar Patil, K.
 
Subject 03 Biochemistry & Molecular Biology
 
Description The recombinases present in the all kingdoms in nature play a crucial role in DNA metabolism processes such as replication, repair, recombination and transcription. However, till date, the role of RecA in the deadly foodborne pathogen Listeria monocytogenes remains unknown. In this study, the authors show that L. monocytogenes expresses recA more than two-fold in vivo upon exposure to the DNA damaging agents, methyl methanesulfonate and ultraviolet radiation. The purified L. monocytogenes RecA protein show robust binding to single stranded DNA. The RecA is capable of forming displacement loop and hydrolyzes ATP, whereas the mutant LmRecAK70A fails to hydrolyze ATP, showing conserved walker A and B motifs. Interestingly, L. monocytogenes RecA and LmRecAK70A perform the DNA strand transfer activity, which is the hallmark feature of RecA protein with an oligonucleotide-based substrate. Notably, L. monocytogenes RecA readily cleaves L. monocytogenes LexA, the SOS regulon and protects the presynaptic filament from the exonuclease I activity. Altogether, this study provides the first detailed characterization of L. monocytogenes RecA and presents important insights into the process of homologous recombination in the gram-positive foodborne bacteria L. monocytogenes.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14281/1/International%20Journal%20of%20Biochemistry%20and%20Cell%20Biology.pdf
Debika, Ojha and Neelakanteshwar Patil, K. (2020) Molecular and functional characterization of the Listeria monocytogenes RecA protein: insights into the homologous recombination process. International Journal of Biochemistry and Cell Biology, 119.