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Comparative assessment of proteins between cell lines and tissues representing carcinomas of the breast using 2-dimensional gel electrophoresis.

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Title Comparative assessment of proteins between cell lines and tissues
representing carcinomas of the breast using 2-dimensional gel
electrophoresis.
 
Creator Venugopal, R. Bovilla
Chaitra, Rai
Mahadevaswamy, G. Kuruburu
SubbaRao, V. Madhunapantula
 
Subject 13 Molecular Biochemistry
 
Description Incidence of breast cancer in the age group of 30 to 50 is increasing at an
alarming rate globally. Moreover, existing treatments are either marginally
effective or resistance developed, hence development of safe and potent pharmacological agents is immediately required. However, establishment of a disease progression marker for early detection, development of safe and effective treatment agents requires identiϑication of key proteins that are exclusively expressed in advanced malignant breast tumors. 2-Dimensional gel
electrophoresis (2-DGE) is one of the early methods used for the identiϑication of deregulated proteins in cancers. Therefore, the proteins of benign and
malignant tumors as well as breast cancer cell lines MCF-7, SKBR-3 and MDAMB-468, were subjected to 2-DGE and relative expression calculated. Analysis of the data showed a signiϑicant increase in the intensity of 7 protein spots
compared to malignant tissues and benign ones. Further, comparison of cell
line proteins with tissue lysates revealed that SKBR-3 is much closer to malignant tumors, hence, may be considered for screening drug targets as well as
for evaluating the efϑicacy of pharmacological agents. In conclusion, our 2-
DGE identiϑied key differences and similarities in the expression of proteins
between breast cancer cell lines, benign and malignant tissues.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14387/1/Int.%20J.%20Res.%20Pharm.%20Sci.%2C%202020%2C%2011%282%29%2C%202294-2302.pdf
Venugopal, R. Bovilla and Chaitra, Rai and Mahadevaswamy, G. Kuruburu and SubbaRao, V. Madhunapantula (2020) Comparative assessment of proteins between cell lines and tissues representing carcinomas of the breast using 2-dimensional gel electrophoresis. International Journal of Research In Pharmaceutical Sciences, 11 (2). pp. 2294-2302.