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Enrichment of Eicosapentaenoic Acid from Sardine Oil with ∆5-Olefinic Bond Specific Lipase from Bacillus licheniformis MTCC 6824

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Relation http://eprints.cmfri.org.in/9952/
 
Title Enrichment of Eicosapentaenoic Acid from Sardine
Oil with ∆5-Olefinic Bond Specific Lipase from
Bacillus licheniformis MTCC 6824
 
Creator Chakraborty, Kajal
Paulraj, R
 
Subject Bioactive compound
 
Description Lipase derived from Bacillus licheniformis MTCC 6824 was purified to homogeneity by anion exchange
chromatography on Amberlite IRA 410 (Cl-) and gel filtration using Sephadex G-100 as judged by
denaturing polyacrylamide gel electrophoresis. The purified lipase was used for hydrolysis of
triacylglycerol in sardine oil to enrich ∆5-polyunsaturated fatty acids (∆5-PUFAs) namely, arachidonic
acid (5,8,11,14-eicosatetraenoic acid, ARA, 20:4n-6) and eicosapentaenoic acid (5,8,11,14,17-
eicosapentaenoic acid, EPA, 20:5n-3). The individual fatty acids were determined as fatty acid methyl
esters (FAMEs) by gas–liquid chromatography and gas chromatography–mass spectroscopy as
FAMEs and N-acyl pyrrolidides. The enzyme exhibited hydrolytic resistance toward ester bonds of
∆5-PUFAs as compared to those of other fatty acids and was proved to be effective for increasing
the concentration of EPA and ARA from sardine oil. Utilizing this fatty acid specificity, EPA and ARA
from sardine oil were enriched by lipase-mediated hydrolysis followed by urea fractionation at 4 °C.
The purified lipase produced the highest degree of hydrolysis for SFAs and MUFAs (81.5 and 72.3%,
respectively, from their initial content in sardine oil) after 9 h. The profile of conversion by lipase
catalysis showed a steady increase up to 6 h and thereafter plateaued down. Lipase-catalyzed
hydrolysis of sardine oil followed by urea adduction with methanol provided free fatty acids containing
55.4% EPA and 5.8% ARA, respectively, after complexation of saturated and less unsaturated fatty
acids. The combination of enzymatic hydrolysis and urea complexation proved to be a promising
method to obtain highly concentrated EPA and ARA from sardine oil.
 
Publisher American Chemical Society
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/9952/1/6.pdf
Chakraborty, Kajal and Paulraj, R (2008) Enrichment of Eicosapentaenoic Acid from Sardine Oil with ∆5-Olefinic Bond Specific Lipase from Bacillus licheniformis MTCC 6824. Journal of Agricultural and Food Chemistry, 55. pp. 1428-1433.