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Antimicrobial potential and seasonality of red algae collected from the southwest coast of India tested against shrimp, human and phytopathogens

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Title Antimicrobial potential and seasonality of red algae collected
from the southwest coast of India tested against shrimp, human
and phytopathogens
 
Creator Manilal, Aseer
Sugathan, Sujith
Seghal Kiran, G
Joseph, Selvin
Shakir, Chippu
Gandhimathi, Ramakrishnan
Lipton, A P
 
Subject Bioactive compound
Algae
 
Description Fifteen seaweeds belong to 13 families and 6 orders of the rhodophyta were sampled for one year from April
2007 to March 2008 along the southwest coast of India (Indian Ocean). The species were examined for in vitro antimicrobial
activity against six pathogenic Vibrio strains isolated from moribund tiger shrimp (Penaeus monodon), six type cultures (Microbial
Type Culture Collection, MTCC) of prominent shrimp Vibrio pathogens, 10 multidrug resistant clinical pathogens, four
species of Candida obtained from pulmonary TB patients and four species of plant pathogenic fungi to evaluate their potency
to be used as natural antibiotics in pharmaceutical and agriculture field. Bioactivity was analyzed from crude extract of fresh
and dried samples prepared from different polar and nonpolar solvents. Of these, four species of red algae (Asparagopsis
taxiformis, Laurencia ceylanica, Laurencia brandenii, Hypnea valentiae) were found to be highly active. Broadest and highest
activity was observed in the crude extract of A. taxiformis. Among the pathogens tested, shrimp pathogenic Vibrios were the
most susceptible organisms while phytopathogens were found to be little resistant. In the present study, methanol was found
to be the best solvent for extracting antimicrobial metabolites from dried samples rather than fresh. Seasonal variation in the
antimicrobial activity was observed with higher level of activity recorded from A. taxiformis between December and January.
The active principle of A. taxiformis was purified in column chromatography, TLC and reverse phase HPLC. The individual
HPLC peaks were subsequently tested against a panel of pathogenic microorganisms and the active constituent was identi-
fied by GC-MS. The antimicrobial profile of A. taxiformis suggested that lipophilic compound which was primarily composed
of pyrrole-2-carboxylic acid, pentadecanoic acid and octadecanoic acid might have functional role in the chemical defence
against microbial invasion and these compounds could be utilized for the development of medically potential products.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/10146/1/Lipton_Annals_of_Microbiology_59_2_207-219_2009.pdf
Manilal, Aseer and Sugathan, Sujith and Seghal Kiran, G and Joseph, Selvin and Shakir, Chippu and Gandhimathi, Ramakrishnan and Lipton, A P (2009) Antimicrobial potential and seasonality of red algae collected from the southwest coast of India tested against shrimp, human and phytopathogens. Annals of Microbiology, 59 (2). pp. 207-219.