ICAR Research Data Repository for Knowledge Management
Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards
CMFRI Repository
View Archive Info| Field | Value | |
| Relation |
http://eprints.cmfri.org.in/7382/
|
|
| Title |
Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards
|
|
| Creator |
Diwan, A D
Joseph, Shoji |
|
| Subject |
Fish Biotechnology
Prawn and Prawn fisheries |
|
| Description |
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. Viable spermatozoa was preserved successfully for a period of 60 days at -3S'C and -196'C temperatures. Among several cryoprotectants used, a combination of DMSO (5%) and glycerol (5%), and DMSO (5%) and trehalose (O.25M) showed best viability rate (75 to 80%) in freeze thawed spermatozoa. Activational changes in spermatozoa during acrosome reaction after induction with egg water have been mentioned. The importance of cryogenic storage of spermatozoa is discussed. |
|
| Date |
2000
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Language |
en
|
|
| Identifier |
http://eprints.cmfri.org.in/7382/1/389-J._OF_AQUACULTURE_IN_THE_LROPICS_2000.pdf
Diwan, A D and Joseph, Shoji (2000) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. Journal of Aquaculture in Tropics, 15 (1). pp. 35-43. |
|