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Characterization of quorum quenching enzymes from endophytic rhizosphere colonizing bacteria.

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Title Characterization of quorum quenching enzymes from endophytic rhizosphere colonizing bacteria.
Not Available
 
Creator Achari, G.A. and Ramesh, R.
 
Subject Quorum quenching Esterase Ralstonia solanacearum Pseudomonas aeruginosa Stenotrophomonas maltophilia
 
Description Not Available
Bacterial wilt caused by Ralstonia solanacearum affects cultivation of solanaceaous vegetables throughout the
world. Quorum quenching is recently emerging as a novel sustainable biocontrol option to curb bacterial diseases
since it does not lead to resistance in the phytopathogen. In the present study, crude quorum quenching
enzymes extracted from endophytic rhizosphere colonizing strains XB7 (Pseudomonas aeruginosa), XB122
(Pseudomonas aeruginosa) and XB102 (Stenotrophomonas maltophilia) were characterized. Vmax/Km ratios indicated
that quorum quenching enzymes had higher activity towards p-Nitrophyenyl acetate as compared to p-
Nitrophyenyl palmitate. Optimum temperature for the activity of quorum quenching esterase of XB7, and XB122
was 37 °C whereas esterase from XB102 had an optimum temperature of 28 °C. Optimum pH for the activity of
quorum quenching enzymes of XB7 and XB122 was pH 6.8 whereas pH 8.0 was optimal for XB102. Cu2+ and
Ca2+ enhanced activity of quorum quenching enzymes extracted from all strains. Activity staining following
native PAGE indicated the molecular weight of esterase from XB7 and XB122 to be approximately 24 kDa
whereas that of XB102 as 20.0 kDa. The study indicates that the extracellular quorum quenching esterases from
XB7, XB122 and XB102 exhibit pH and temperature optima similar to common soil enzymes and can remain
active in presence of metal ions which can occur in rhizosphere soil. These features of extracellular quorum
quenching esterases from endophytic and rhizosphere colonizing strains led us to a conclusion that these enzymes
may have potential applications in innovative and ecofriendly bacterial wilt management approaches.
Indian Council of Agricultural Research, New Delhi , India through “Outreach project on Phytophthora, Fusarium and Ralstonia diseases of horticultural and field crops”- (PhytoFuRa).
 
Date 2018-11-09T08:49:36Z
2018-11-09T08:49:36Z
2017-11-13
 
Type Research Paper
 
Identifier Achari, G.A. and Ramesh, R. 2018. Characterization of quorum quenching enzymes from endophytic rhizosphere colonizing bacteria. Biocatalysis and Agricultural Biotechnology, 13: 20-24.
1878-8181
http://krishi.icar.gov.in/jspui/handle/123456789/9948
 
Language English
 
Relation Not Available;
 
Publisher Elsevier