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Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea wall

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Title Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea wall
Not Available
 
Creator Ashwani Kumar, Suresh Chand Goyal, Charu Lata, Neelam Sharma, Pooja Dhansu and Jagdish Parshad
 
Subject Dioscorea deltoidea,Revised tobacco medium, Root tuber, Indirect regeneration, Direct regeneration
 
Description Not Available
The present study was explained about a direct
and indirect regeneration protocol for an important wild
medicinal plant Dioscorea deltoidea, a rich source of
saponins and diosgenin. It was found that source of
explant, basal medium composition, concentration of plant
growth regulator and the photoperiod directly control
morphogenetic responses. Out of all explants tested, only
nodal segments performed better on the experimental
growth media. Nodal segments initiated direct organogenesis
when cultured on MS and RT (revised tobacco)
medium supplemented with BAP and NAA or IBA.
Growth medium supplemented with BAP (1.5 mg/l) and
IBA (0.5 mg/l) induced maximum regeneration. Indirect
roots regeneration was achieved on RT medium supplemented
with IAA 1.0 mg/l and RT medium supplemented
with BAP 1.0 mg/l and NAA 0.5 mg/l induced shoot
regeneration through callus formed on MS medium containing
2, 4-D (2.0 mg/l).
Not Available
 
Date 2018-11-14T11:40:16Z
2018-11-14T11:40:16Z
2017-08-01
 
Type Book
 
Identifier Ashwani Kumar, Suresh Chand Goyal, Charu Lata, Neelam Sharma, Pooja Dhansu and Jagdish Parshad (2017) Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea wall. National Academy Science Letters. 40(4):237–240
Not Available
http://krishi.icar.gov.in/Publication/handle/123456789/11303
 
Language English
 
Relation Not Available;
 
Publisher Springer