Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea wall
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Title |
Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea wall
Not Available |
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Creator |
Ashwani Kumar, Suresh Chand Goyal, Charu Lata, Neelam Sharma, Pooja Dhansu and Jagdish Parshad
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Subject |
Dioscorea deltoidea,Revised tobacco medium, Root tuber, Indirect regeneration, Direct regeneration
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Description |
Not Available
The present study was explained about a direct and indirect regeneration protocol for an important wild medicinal plant Dioscorea deltoidea, a rich source of saponins and diosgenin. It was found that source of explant, basal medium composition, concentration of plant growth regulator and the photoperiod directly control morphogenetic responses. Out of all explants tested, only nodal segments performed better on the experimental growth media. Nodal segments initiated direct organogenesis when cultured on MS and RT (revised tobacco) medium supplemented with BAP and NAA or IBA. Growth medium supplemented with BAP (1.5 mg/l) and IBA (0.5 mg/l) induced maximum regeneration. Indirect roots regeneration was achieved on RT medium supplemented with IAA 1.0 mg/l and RT medium supplemented with BAP 1.0 mg/l and NAA 0.5 mg/l induced shoot regeneration through callus formed on MS medium containing 2, 4-D (2.0 mg/l). Not Available |
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Date |
2018-11-14T11:40:16Z
2018-11-14T11:40:16Z 2017-08-01 |
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Type |
Book
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Identifier |
Ashwani Kumar, Suresh Chand Goyal, Charu Lata, Neelam Sharma, Pooja Dhansu and Jagdish Parshad (2017) Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea wall. National Academy Science Letters. 40(4):237–240
Not Available http://krishi.icar.gov.in/Publication/handle/123456789/11303 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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