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Biochemical Studies During Sequential stages of Root and Shoot Differentiation in Callus cultures of Cardiospermum halicacabum L

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Title Biochemical Studies During Sequential stages of Root and Shoot Differentiation in Callus cultures of Cardiospermum halicacabum L
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Creator Ashwani Kumar, Goyal S C, Pooja, Lata C and Parshad J
 
Subject Ballon vine, callus, metabolites, enzymes, differentiation
 
Description Not Available
Present investigation was carried out on
Cardiospermum halicacabum L. (Ballon Vine) -
an important medicinal plant. Leaf explants were
found to be the best for induction and growth of
callus. Depending on fresh and dry weight of
callus, high growth value callus was obtained
on MS medium supplemented with 2, 4-D (1.0
mg/l) in combination with Kn (1.0 mg/l). This
callus was selected for further studies of root and
shoot organogenesis as well as biochemical
studies. Regeneration of roots (15 days) from
callus was observed on MS medium + BAP (0.5
mg/l) + NAA (0.3 mg/l) while shoots (16 days) on
MS medium + BAP (0.5 mg/l) + NAA (0.1 mg/l).
Starch content and reducing sugars were high in
control or undifferentiated callus, which further
increases significantly in root and shoot
differentiating cultures. Contents of total soluble
sugars, total soluble proteins and total phenols
were lower in the control callus which increases
in the root and shoot differentiating cultures. The
activities of enzymes i.e. a-amylase, acid
protease and peroxidase increased up to
appearance of green patches (8th day) and were
reached at peak on 12th day, the day that coincide
with the visual appearance of roots and shoot.
Conversely, the acid invertase activity decreases
till the appearance of roots and shoots.
Not Available
 
Date 2018-11-14T11:39:38Z
2018-11-14T11:39:38Z
2015-10-01
 
Type Research Paper
 
Identifier Ashwani Kumar, Goyal S C, Pooja, Lata C and Parshad J (2015) Biochemical studies during sequential stages of root and shoot differentiation in callus cultures of Cardiospermum halicacabum L. Current Trends in Biotechnology and Pharmacy. 9 (4): 380-388
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http://krishi.icar.gov.in/Publication/handle/123456789/11300
 
Language English
 
Relation Not Available;
 
Publisher Not Available