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Plastid transformation in eggplant-Solanum melongena. Methods in Molecular Biology

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Title Plastid transformation in eggplant-Solanum melongena. Methods in Molecular Biology
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Creator Bansal KC, Singh AK
 
Subject Aminoglycoside 3"-adenyltransferase, Eggplant, Plastid transformation, Solanum melongena
 
Description Not Available
Eggplant ( Solanum melongena L.) is an important vegetable crop of tropical and temperate regions of the
world. Here we describe a procedure for eggplant plastid transformation, which involves preparation of
explants, biolistic delivery of plastid transformation vector into green stem segments, selection procedure,
and identifi cation of the transplastomic plants. Shoot buds appear from cut ends of the stem explants following 5–6 weeks of spectinomycin selection after bombardment with the plastid transformation vector containing aadA gene as selectable marker. Transplastomic lines are obtained after the regenerated shoots are subjected to several rounds of spectinomycin selection over a period of 9 weeks. Homoplasmic transplastomic lines are further confi rmed by spectinomycin and streptomycin double selection. The transplastomic technology development in this plant species will open up exciting possibilities for improving crop performance, metabolic engineering, and the use of plants as factories for producing biopharmaceuticals.
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Date 2018-12-17T08:28:47Z
2018-12-17T08:28:47Z
2014
 
Type Book chapter
 
Identifier Not Available
978-1-62703-994-9
http://krishi.icar.gov.in/jspui/handle/123456789/16032
 
Language English
 
Relation Not Available;
 
Publisher Springer