Plastid transformation in eggplant-Solanum melongena. Methods in Molecular Biology
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Title |
Plastid transformation in eggplant-Solanum melongena. Methods in Molecular Biology
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Creator |
Bansal KC, Singh AK
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Subject |
Aminoglycoside 3"-adenyltransferase, Eggplant, Plastid transformation, Solanum melongena
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Description |
Not Available
Eggplant ( Solanum melongena L.) is an important vegetable crop of tropical and temperate regions of the world. Here we describe a procedure for eggplant plastid transformation, which involves preparation of explants, biolistic delivery of plastid transformation vector into green stem segments, selection procedure, and identifi cation of the transplastomic plants. Shoot buds appear from cut ends of the stem explants following 5–6 weeks of spectinomycin selection after bombardment with the plastid transformation vector containing aadA gene as selectable marker. Transplastomic lines are obtained after the regenerated shoots are subjected to several rounds of spectinomycin selection over a period of 9 weeks. Homoplasmic transplastomic lines are further confi rmed by spectinomycin and streptomycin double selection. The transplastomic technology development in this plant species will open up exciting possibilities for improving crop performance, metabolic engineering, and the use of plants as factories for producing biopharmaceuticals. Not Available |
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Date |
2018-12-17T08:28:47Z
2018-12-17T08:28:47Z 2014 |
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Type |
Book chapter
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Identifier |
Not Available
978-1-62703-994-9 http://krishi.icar.gov.in/jspui/handle/123456789/16032 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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