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Bacterial diversity in the rumen of mithun (Bos frontalis) fed on mixed tree leaves and rice straw based diet

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Title Bacterial diversity in the rumen of mithun (Bos frontalis) fed on mixed tree leaves and rice straw based diet
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Creator Not Available
K. C. Das
S. S. Paul
L. Sahoo
K. K. Baruah,
P. K. Subudhi
K. Ltu
C. Rajkhowa
 
Subject Tree leaves, bacteria, clones, sequences.
 
Description Not Available
This work was done to study the bacterial diversity of mithun (Bos frontalis) fed on mixed tree leaves
and rice straw based diet. Genomic DNA was extracted from the rumen liquor of mithun, 16S rDNA
sequences were amplified, cloned and randomly selected for sequencing. The nearest neighbors were
retrieved from the NCBI through a BLAST search and a phylogenetic tree was constructed. In our
findings, 12% of clones showed similarity with known bacterial species (Prevotella ruminicola,
Butyrivibriofibrisolvens,Pseudobutyrivibrioruminis,Succinivibriodextrinosolvensand
Ruminococcus flavefaciens) and 6% of clones showed similarity with known bacterial genus
(Butyrivibrio species, Streptococcus species) of 97-100% similarities. Twenty-two percent of clones
showed similarity with known bacteria (P. ruminicola, Prevotella species, Sporanaerobacter acetigenes,
Clostridiales bacterium, Bacteroidetes bacterium) of 90-97% similarities. Sequences of all the clones
were also classified by using taxonomic classifier software available at Ribosomal Database Project
and classification showed that, all the clones were under four phyla, namely Bacteroidetes (54%),
Firmicutes (36%), Proteobacteria (4%) and Tenericutes (2%). The experiment showed that, bacterial
population in the rumen of mithun fed on mixed tree leaves and rice straw based diet harbor diversified
species of bacteria responsible for lignocellulosic feedstuffs.
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Date 2019-01-31T09:56:04Z
2019-01-31T09:56:04Z
2014-03-26
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/16658
 
Language English
 
Relation Not Available;
 
Publisher African Journal of Microbiology Research