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Characterization and Gene Expression Profiling of Epididymal Sperm Collected from Dead Mithun (Bos Frontalis) Bulls and its Preservation

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Title Characterization and Gene Expression Profiling of Epididymal Sperm Collected from Dead Mithun (Bos Frontalis) Bulls and its Preservation
Not Available
 
Creator Not Available
M. Mondal
KK. Baruah
A. Chatterjee
MK. Ghosh
 
Subject Epididymal sperm; Mithun (Bos frontalis); Cryopreservation; Conservation; Fertility; Gene expression.
 
Description Not Available
Conserving the mithun (Bos frontalis), a rare bovine of south-east
Asia, could benefit from effective ex situ genetic management and
breeding programmes including the use of assisted reproduction. Longterm
storage of epididymal sperm collected from dead mithun bulls by
cryopreservation, with high survival rate, is essential for the
establishment of genetic resource banks of this rare species. A study
was therefore conducted to (i) characterize the epididymal sperm
collected from dead mithun bulls, (ii) investigate the effectiveness of
epididymal sperm for cryopreservation and (iii) to study the expression
pattern of genes related to motility (TSSK6 and ADAM5P ) and
fertility (PRM1, PRM2, PRM3, Tnp1 and Tnp2) in mithun epididymal
sperm. For the purpose, sperm collected from caudal epididymis of
eight dead mithun bulls were evaluated for concentration, progressive
motility, morphological abnormalities, live sperm counts, acrosome
integrity, membrane stability (hypo-osmotic swelling test; HOST) and
DNA integrity. Epididymal sperm were cryopreserved using tris-egg
yolk-glycerol diluent in liquid nitrogen. Post-thaw quality of the
cryopreserved sperm in terms of progressive motility, morphological
abnormalities, live cell counts, acrosome integrity, membrane stability
and DNA integrity were assessed. The RNA extracted from fresh and
post-thawed cryopreserved epididymal sperm was reverse transcribed
to cDNA and expressions of the genes related to motility and fertility
were determined by RT-PCR. The progressive motility, live cell counts,
morphological abnormalities and acrosome integrity (normal)
of fresh sperm were 89.9±2.7, 88.7±6.7, 7.8±1.07 and 95.3±7.8%,
respectively. Fresh sperm that responded to HOST were 88.3±7.2%,
and 89.5±6.4% fresh sperm had intact DNA. Following
cryopreservation, the percentage of progressive motility (fresh vs
frozen-thawed), live cell counts, morphological abnormalities,
acrosome integrity, membrane stability and DNA integrity were found
to decrease significantly (P
Not Available
 
Date 2019-03-20T09:31:26Z
2019-03-20T09:31:26Z
2013
 
Type Research Paper
 
Identifier Not Available
2231-1238
http://krishi.icar.gov.in/jspui/handle/123456789/17552
 
Language English
 
Relation Not Available;
 
Publisher Research India Publications