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Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers

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Title Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers
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Creator O. K. Raina, Siju Susan Jacob, M. Sankar, D. Bhattacharya, S. Bandyopadyay, Anju Varghese, Jayanta Kumar Chamuah & H. Lalrinkima
 
Subject Fasciola gigantica 28S ribosomal DNA ITS-2 Genetic characterization
 
Description Not Available
Ribosomal DNA sequences of the second
internal transcribed spacer (ITS-2) and 28S ribosomal
DNA (618 bp) of Fasciola gigantica collected from cattle
and buffaloes from four different geographical locations of
India, were characterized for genotyping. ITS-2 sequence
was analyzed in 28 worms that was typical of F. gigantica
and differed at six positions, with one of these being
a distinguishing deletion (T) at the 327th position in
F. gigantica relative to F. hepatica. However, Fasciola
specimens also showed intraspecies sequence polymorphism
in the ITS-2, with two different ITS-2 sequences
existing in the ribosomal DNA (rDNA) array within a
single Fasciola worm. One of the sequences was identical
to that of F. gigantica and the other showed extensive
sequence polymorphism in the ITS-2. Using BspH1-
restriction fragment length polymorphism, six variable
ITS-2 sequences in F. gigantica were identified within
these parasite specimens and were found distributed in
these four geographical regions. 28S rDNA sequence of 24
flukes, collected from the above four geographical regions,
showed a single nucleotide polymorphism at 284th nucleotide
(G/A). Analyzing the sequence data of 28S rDNA of
F. gigantica available from some African and Asian
countries for this polymorphic 284th nucleotide position, it
is proposed that there are two basic lineages of the F.
gigantica for 28S rDNA existing in the fluke populations
from five African and several Asian countries.
Not Available
 
Date 2019-11-11T05:17:30Z
2019-11-11T05:17:30Z
2013-02-24
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/24624
 
Language English
 
Relation Not Available;
 
Publisher Not Available