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Molecular identification of Mecistocirrus digitatus and Toxocara vitulorum in the mithun (Bos frontalis) from north-east India

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Title Molecular identification of Mecistocirrus digitatus and Toxocara vitulorum in the mithun (Bos frontalis) from north-east India
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Creator Jayanta Kumar Chamuah1 • O. K. Raina2 • A. Sakhrie1 • N. Gama3
 
Subject Mithun . Parasites. Internal transcribed spacers. Beta tubulin gene
 
Description Not Available
Mecistocirrus digitatus and Toxocara vitulorum
are common pathogenic nematode parasites of mithun (Bos
frontalis). Species identification by morphological features
was confirmed by molecular identification of M. digitatus
and T. vitulorum. The internal transcribed spacer-2 (ITS-2)
region and beta tubulin gene of M. digitatus were polymerase
chain reaction (PCR) amplified and sequenced.
ITS-2 sequence analysis showed 100% homology with
other isolates of M. digitatus and 83% identity with Haemonchus
contortus and H. placei, respectively. Likewise,
ITS-1 and ITS-2 sequences of T. vitulorum were PCR
amplified and sequenced. Sequence analysis of these
internal transcribed spacers from five worms of the parasite
from mithun showed no intraspecific variations with T.
vitulorum isolates from domestic ruminants.
Not Available
 
Date 2019-11-11T05:18:01Z
2019-11-11T05:18:01Z
2017-01-10
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/24627
 
Language English
 
Relation Not Available;
 
Publisher Not Available