Cloning and expression of Toxocara canis arginine kinase recombinant protein for serodetection of toxocarosis in dogs
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Title |
Cloning and expression of Toxocara canis arginine kinase recombinant protein for serodetection of toxocarosis in dogs
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Creator |
Varsha Bagde, Siju Susan Jacob, S. Samanta, Shivani Sahu, N.R., Sudhakar, J.K. Chamuah, Harikesh Singh Yadav, Rajat Garg and O.K. Raina*
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Subject |
Toxocara canis, Dog, Arginine kinase, Recombinant antigen, ELISA, Serodiagnosis.
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Description |
Not Available
Toxocarosis is a widespread zoonosis caused by the ascarid Toxocara canis. Serodiagnosis of toxocarosis is carried out using Toxocara excretory-secretory and recombinant antigens. In the present study T. canis arginine kinase gene was amplified by polymerase chain reaction, cloned in a prokaryotic expression vector and expressed in Escherichia coli. The recombinant protein was used in IgG-ELISA for detection of T. canis infection in the adult dogs. Further evaluation of this recombinant antigen in the diagnosis of T. canis infection in dogs is under way. Not Available |
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Date |
2019-11-11T05:19:04Z
2019-11-11T05:19:04Z 2013-01-01 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/24634 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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