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Cloning and expression of Toxocara canis arginine kinase recombinant protein for serodetection of toxocarosis in dogs

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Title Cloning and expression of Toxocara canis arginine kinase recombinant protein for serodetection of toxocarosis in dogs
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Creator Varsha Bagde, Siju Susan Jacob, S. Samanta, Shivani Sahu, N.R., Sudhakar, J.K. Chamuah, Harikesh Singh Yadav, Rajat Garg and O.K. Raina*
 
Subject Toxocara canis, Dog, Arginine kinase, Recombinant antigen, ELISA, Serodiagnosis.
 
Description Not Available
Toxocarosis is a widespread zoonosis caused by the ascarid Toxocara canis. Serodiagnosis
of toxocarosis is carried out using Toxocara excretory-secretory and recombinant antigens. In
the present study T. canis arginine kinase gene was amplified by polymerase chain reaction,
cloned in a prokaryotic expression vector and expressed in Escherichia coli. The recombinant
protein was used in IgG-ELISA for detection of T. canis infection in the adult dogs. Further
evaluation of this recombinant antigen in the diagnosis of T. canis infection in dogs is under
way.
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Date 2019-11-11T05:19:04Z
2019-11-11T05:19:04Z
2013-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/24634
 
Language English
 
Relation Not Available;
 
Publisher Not Available