Record Details

Current status of sperm functional genomics and its diagnostic potential of fertility in bovine (Bos taurus).

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Current status of sperm functional genomics and its diagnostic potential of fertility in bovine (Bos taurus).
Not Available
 
Creator Selvaraju S, Parthipan S, Somashekar L, Krishnan BB, Kolte AP, Arangasamy A, Ravindra JP and Krawetz SA.
 
Subject Bull, spermatozoal RNA, semen quality, cryopreservation, fertility
 
Description Not Available
With artificial insemination (AI) and other precision dependent assisted reproductive technologies (ART) being followed in large scale in human and animal reproduction, assessing semen quality and fertilizability is under continuous scrutiny. Various tests have been developed to predict semen quality, but so far no single, highly reliable test is available. In this regard, transcriptomic profiling of spermatozoa assumes significance as it carries the information about spermatogenesis, sperm function, and paternal roles in post-fertilization events. Human spermatozoal transcriptome profiling has been carried out on a large number of individuals to predict the semen quality. A study in human indicated that the outcome of some idiopathic couples seeking reproductive care could be helped using transcriptomic profiling of spermatozoa. Such studies have a direct impact on the bovine dairy industry, wherein AI is practiced. Limited studies in bovine spermatozoal transcriptome profiling have revealed that the spermatozoa contain various classes of RNA, like in human. Approximately 13,000 bovine genes yield a series of spermatozoal transcripts, of which most are fragmented in nature. Their abundance is indicative of the timing of events associated with spermatogenesis, e.g., PRM1, IGF1, BMP2; sperm function, TSSK6, CRISP, HSFY2; fertility, UBE2D3, Integrin-β, LDC-1; and embryonic development, miR34c-5p, BCL2L11, BRCA1. The most abundant translated bovine transcripts are BSP3 and SPATA18, and are involved in regulation of germ cell development and the maintenance of chromatin integrity during spermatogenesis respectively. The presence of transcripts associated with placental development, e.g., placental associated glycoproteins (PAGs) have suggested their possible influence beyond early embryonic development. Changes in transcript levels like RPL31 and PRKCE that increase, and PRM1 that decreases, during cryopreservation need to be defined in order to optimize cryopreservation and fertility yield. Spermatozoal transcriptome profiling with validation studies are warranted in large numbers of animals to elucidate their significance for selecting fertile bulls for the breeding program.
Not Available
 
Date 2019-12-02T12:29:27Z
2019-12-02T12:29:27Z
2018-03-14
 
Type Article
 
Identifier Selvaraju S, Parthipan S, Somashekar L, Krishnan BB, Kolte AP, Arangasamy A, Ravindra JP and Krawetz SA. 2018. Current status of sperm functional genomics and its diagnostic potential of fertility in bovine (Bos taurus). Systems Biology in Reproductive Medicine, 14:1-18.
1939-6376 (print); 1939-6368 (web)
http://krishi.icar.gov.in/jspui/handle/123456789/26427
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis