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Production of sheep embryos in vitro

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Title Production of sheep embryos in vitro
Not Available
 
Creator Mor A, Mondal S and Reddy IJ
 
Subject Oocytes; Sheep; In vitro maturation; In vitro fertilization; Embryo
 
Description Not Available
In-Vitro Maturation (IVM) and In-Vitro Fertilization (IVF) are the potential
methods for producing the early embryos in large numbers. Recently many
producers have introduced IVF into their reproductive programs. Successful
in vitro production of embryo depends upon the good oocyte maturation
and correct sperm capacitation. Ovaries were collected from non-pregnant
slaughtered sheep from a local slaughterhouse in normal saline. Ovaries having
visible follicle and granular homogenous ooplasm were selected and cultured
in maturation medium at 38.5º
C, 5% CO2
and 95% humidity for 22-24 hr. The
degree of cumulus cell expansion was determined after 22-24 hr of IVM and
oocyte with expanded cumulus cell mass to at least 2 diameters away from the
zona pellucida were considered as cumulus expanded. The matured oocytes
were inseminated with 1 to 2 million spermatozoa/mL in Brackett and Oliphant
medium and the embryos were then cultured in CO2
incubator at 38.5°
C, 5%
CO2
and 95% humidity and the cleavage was checked after 42-48 hr post
insemination. The embryos were then further cultured for 6-7 days in order to
produce a complete developmental series from the 2 cell to blastula stage of
embryonic development. Our results showed that the percentage of maturation
was 81.81±1.10, cleavage rate was 66.03±1.33, 8-16 cells was 43.44±0.62,
Morula was 20.01±0.29, and Blastula was 8.59±0.56. The maturation rate was
significantly (P
Not Available
 
Date 2019-12-04T10:53:20Z
2019-12-04T10:53:20Z
2017-07-24
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/27341
 
Language English
 
Relation Not Available;
 
Publisher Austin publishing group