Production of sheep embryos in vitro
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Title |
Production of sheep embryos in vitro
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Creator |
Mor A, Mondal S and Reddy IJ
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Subject |
Oocytes; Sheep; In vitro maturation; In vitro fertilization; Embryo
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Description |
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In-Vitro Maturation (IVM) and In-Vitro Fertilization (IVF) are the potential methods for producing the early embryos in large numbers. Recently many producers have introduced IVF into their reproductive programs. Successful in vitro production of embryo depends upon the good oocyte maturation and correct sperm capacitation. Ovaries were collected from non-pregnant slaughtered sheep from a local slaughterhouse in normal saline. Ovaries having visible follicle and granular homogenous ooplasm were selected and cultured in maturation medium at 38.5º C, 5% CO2 and 95% humidity for 22-24 hr. The degree of cumulus cell expansion was determined after 22-24 hr of IVM and oocyte with expanded cumulus cell mass to at least 2 diameters away from the zona pellucida were considered as cumulus expanded. The matured oocytes were inseminated with 1 to 2 million spermatozoa/mL in Brackett and Oliphant medium and the embryos were then cultured in CO2 incubator at 38.5° C, 5% CO2 and 95% humidity and the cleavage was checked after 42-48 hr post insemination. The embryos were then further cultured for 6-7 days in order to produce a complete developmental series from the 2 cell to blastula stage of embryonic development. Our results showed that the percentage of maturation was 81.81±1.10, cleavage rate was 66.03±1.33, 8-16 cells was 43.44±0.62, Morula was 20.01±0.29, and Blastula was 8.59±0.56. The maturation rate was significantly (P Not Available |
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Date |
2019-12-04T10:53:20Z
2019-12-04T10:53:20Z 2017-07-24 |
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Type |
Article
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/27341 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Austin publishing group
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