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Comparaive analysis of silencing expression of myostatin (MSTN) and its two receptors (ACVR2A and ACVR2B) genes affecting growth traits in knock down chicken

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Title Comparaive analysis of silencing expression of myostatin (MSTN) and its two receptors (ACVR2A and ACVR2B) genes affecting growth traits in knock down chicken
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Creator Bhattacharya, T.K., Shukla, R., Chatterjee, R.N. and Bhanja, S.K.
 
Subject MSTN, ACVR2A, ACVR2B, tripple knock down, chicken
 
Description Not Available
Myostatin (MSTN), a growth differentiation factor-8 regulates muscular development through its
receptors, ACVR2A (Activin receptor type IIA) and ACVR2B (Activin receptor type IIB) by inhibiting
cellular differentiation of developing somites during embryonic stage and diminishing myofibriller
growth during post-embryonic period. The objective of this study was to compare the effect of
knockdown of expression of myostatin, ACVR2A and ACVR2B genes on growth traits in chicken.
The shRNAs for Myostatin, ACVR2A and ACVR2B genes were designed, synthesized and cloned in
DEST vector. The recombinant molecules were transfected into the spermatozoa and transfected
spermatozoa were inseminated artificially to the hens to obtain fertile eggs. The fertile eggs were
collected, incubated in the incubator and hatched to chicks. Silencing of ACVR2B gene showed
significantly higher body weight than other single, double and triple knock down of genes in transgenic
birds. The carcass traits such as dressing%, leg muscle%, and breast muscle% were found with the
highest magnitudes in birds with silencing of the ACVR2B gene as compared to the birds with that of
other genes and control group. The lowest serum cholesterol and HDL content was found in ACVR2B
silencing birds. The total RBC count was the highest in this group though the differential counts did not
differ significantly among various silencing and control groups of birds. It is concluded that silencing of
only one receptor of MSTN particularly, ACVR2B may augment the highest growth in chicken during
juvenile stage. Our findings may be used as model for improving growth in other food animals and
repairing muscular degenerative disorders in human and other animals.
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Date 2019-12-04T11:08:05Z
2019-12-04T11:08:05Z
2019-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/27375
 
Language English
 
Relation Not Available;
 
Publisher Not Available