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Technologies for identification of species and sex of meat

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Title Technologies for identification of species and sex of meat
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Creator Dr.Girish Patil,S., Dr.A.R.Sen., Dr.S.Vathiyanathan., DrB.M.Naveen., Dr.M.Muthukumar., Dr.V.V.Kulkarni.
 
Subject Selective non vegetarianism is a global phenomenon.Preferences of consumer vary between meats originated from different species and such preferences are often influenced by religious sentiments.
 
Description Not Available
We developed a polymerase chain reaction (PCR)-based molecular method for sexing and identification using sexual dimorphism between the Zinc Finger-X and -Y (ZFX-ZFY) gene and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for mitochondrial DNA (mtDNA) cytochrome B (CYTB) gene in meat pieces and commercial sausages from animals of different origins. Sexual dimorphism based on the presence or absence of SINE-like sequence between ZFX and ZFY genes showed distinguishable band patterns between male and female DNA samples and were easily detected by PCR analyses. Male DNA had two PCR products appearing as distinct two bands (ZFX and ZFY), and female DNA had a single band (ZFX). Molecular identification was carried out using PCR-RFLP of CYTB gene, and showed clear species classification results. The results yielded identical information on the sexes and the species of the meat samples collected from providers without any records. The analyses for DNA isolated from commercial sausage showed that pig was the major source but several sausages originated from chicken and Atlantic cod. Applying this PCR-based molecular method was useful and yielded clear sex information and identified the species of various tissue samples originating from livestock
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Date 2019-12-05T10:53:41Z
2019-12-05T10:53:41Z
2014-01-01
 
Type Technical Report
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/28157
 
Language English
 
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Publisher Not Available