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Molecular characterization and phylogenetic analysis of Trypanosoma evansi from Northern India based on 18S ribosomal gene

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Title Molecular characterization and phylogenetic analysis of Trypanosoma evansi from Northern India based on 18S ribosomal gene
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Creator Rajender Kumar, S.P. Sarkhel, Saroj Kumar, Kanisht Batra, Khushboo Sethi, Shikha Jain, Sanjay Kumar, B.N. Tripathi
 
Subject Surra Trypanosoma evansi 18S rRNA gene Genetic diversity
 
Description Not Available
Six Trypanosoma evansi isolates were collected from ponies (PH1 and PK6), camel (CB2), donkeys (DJ3 and DH4)
and cattle (CK5) from different States of Northern India (Haryana, Rajasthan, Uttar Pradesh and Gujarat) for
molecular characterization based on 18S rRNA gene. The 18S rRNA gene (2251 bp) of different isolates was
amplified, cloned and custom sequenced separately. Based on sequence and phylogenetic analysis of all six
isolates, collected from different hosts as well as geographical areas, were having high identity among Indian T.
evansi strains (99.7%) and with other strains of T. evansi (99.2%) distributed worldwide. There is less genetic
diversity among different salivarian strains of T. evansi except few nucleotide changes at significant locations in
one Indian isolate of camel origin (CB2). All Indian T. evansi isolates were grouped in salivarian clade with high
bootstrap values and remained far away from stercorarian clade having 88–90% nucleotide identity. The study
will be helpful in understanding the evolutionary relationship, molecular epidemiology and variation in disease
pathogenesis among different T. evansi strains. Further, more studies are required on large number of isolates
collected from diverse host and geographical areas to reaffirm the present finding.
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Date 2019-12-06T07:26:23Z
2019-12-06T07:26:23Z
2018-12-25
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/28417
 
Language English
 
Relation Not Available;
 
Publisher Elsevier