Optimization of protein isolation and comparative proteomics of pathogenic Fusarium oxysporum f. sp. cubense (P-Foc) and non pathogenic Fusarium oxysporum (np-Fo).
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Title |
Optimization of protein isolation and comparative proteomics of pathogenic Fusarium oxysporum f. sp. cubense (P-Foc) and non pathogenic Fusarium oxysporum (np-Fo).
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Creator |
Kalai Ponmani , K., Thangavelu, R., and Varun, G.
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Subject |
Fusarium oxysporum f. sp. cubense, bananas, gels, gene expression regulation, mycelium, pathogen survival, pathogenicity, proteins, proteome, proteomics, signal transduction, vascular wilt
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Description |
Not Available
Panama wilt of banana caused by F. oxysporum f. sp. cubense (Foc) is a devastating disease of banana and in severe condition causes 100% yield loss. In order to understand the molecular mechanism of pathogenicity for the effective management of the wilt disease, three protocols (MgCl2/CHAPS, TCA-Acetone and TCA/Phenol-SDS) were evaluated to extract protein from Foc mycelial tissue. Comparatively, TCA/Phenol-SDS method of protein precipitation and extraction resulted in maximum protein quantity (0.76±0.025 mg/g of mycelial tissue) and ca.600 spots were identified in CBB stained gels. A comparative proteomic study conducted between pathogenic F. oxysporum f. sp. cubense (P-Foc) (race 1, VCG 0124) and non-pathogenic Fusarium oxysporum (np-Fo) revealed a difference at the proteome level. In the comparative proteomics study, 23 spots were found to be differentially expressed with ≥ 1.5 fold intensity differences between P-Foc and np-Fo. Preliminarily, six spots (5 significantly upregulated and 1 unique proteins) from P-Foc was randomly picked and subjected to MALDITOF/MS analysis. Further, MASCOT search and functional annotation revealed that these up regulated proteins identified in P-Foc may be involved in invasive growth, signal transduction and pathogen persistence during adverse condition. ICAR |
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Date |
2018-07-09T04:28:20Z
2018-07-09T04:28:20Z 2017-07-01 |
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Type |
Research Paper
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Identifier |
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/6115 |
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Language |
English
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Relation |
Not Available;
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Publisher |
EDIZIONI ETS, PIAZZA CARRARA 16-19, 56126 PISA, ITALY
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