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Optimization of protein isolation and comparative proteomics of pathogenic Fusarium oxysporum f. sp. cubense (P-Foc) and non pathogenic Fusarium oxysporum (np-Fo).

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Title Optimization of protein isolation and comparative proteomics of pathogenic Fusarium oxysporum f. sp. cubense (P-Foc) and non pathogenic Fusarium oxysporum (np-Fo).
 
Creator Kalai Ponmani , K., Thangavelu, R., and Varun, G.
 
Subject Fusarium oxysporum f. sp. cubense, bananas, gels, gene expression regulation, mycelium, pathogen survival, pathogenicity, proteins, proteome, proteomics, signal transduction, vascular wilt
 
Description Not Available
Panama wilt of banana caused by F. oxysporum f. sp. cubense (Foc) is a devastating disease of banana and in severe condition causes 100% yield loss. In order to understand the molecular mechanism of pathogenicity for the effective management of the wilt disease, three protocols (MgCl2/CHAPS, TCA-Acetone and TCA/Phenol-SDS) were evaluated to extract protein from Foc mycelial tissue. Comparatively, TCA/Phenol-SDS method of protein precipitation and extraction resulted in maximum protein quantity (0.76±0.025 mg/g of mycelial tissue) and ca.600 spots were identified in CBB stained gels. A comparative proteomic study conducted between pathogenic F. oxysporum f. sp. cubense (P-Foc) (race 1, VCG 0124) and non-pathogenic Fusarium oxysporum (np-Fo) revealed a difference at the proteome level. In the comparative proteomics study, 23 spots were found to be differentially expressed with ≥ 1.5 fold intensity differences between P-Foc and np-Fo. Preliminarily, six spots (5 significantly upregulated and 1 unique proteins) from P-Foc was randomly picked and subjected to MALDITOF/MS analysis. Further, MASCOT search and functional annotation revealed that these up regulated proteins identified in P-Foc may be involved in invasive growth, signal transduction and pathogen persistence during adverse condition.
ICAR
 
Date 2018-07-09T04:28:20Z
2018-07-09T04:28:20Z
2017-07-01
 
Type Research Paper
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/6115
 
Language English
 
Relation Not Available;
 
Publisher EDIZIONI ETS, PIAZZA CARRARA 16-19, 56126 PISA, ITALY