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Construction of BBTV rep gene RNAi vector and evaluate the silencing mechanism through injection of Agrobacterium tumefaciens transient expression system in BBTV infected hill banana plants cv. Virupakshi (AAB).

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Title Construction of BBTV rep gene RNAi vector and evaluate the silencing mechanism through injection of Agrobacterium tumefaciens transient expression system in BBTV infected hill banana plants cv. Virupakshi (AAB).
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Creator Elayabalan, S., Subramaniam, S. and Selvarajan, R.
 
Subject RNAi-BBTV rep, Hill banana, Pathogen-derived resistance
 
Description Not Available
One of the most severe viral diseases of banana worldwide is caused by banana bunchy top virus (BBTV). It is a nanovirus transmitted by the aphids Pentalonia nigronervosa. Currently, there are no strategies available in order to completely protect bananas against the BBTV virus. The improvement of banana resistance to BBTV disease through conventional breeding has not been successful and therefore an alternative approach such as genetic engineering system requires producing resistance banana cultivar. In this study, gene isolation and construction of RNAi vector for bunchy top disease management in hill banana was determined. The target of the RNAi is the Rep protein encoded by the BBTV-DNA1. It can be concluded that the isolated rep gene displayed 100% sequence identity to the BBTV rep gene of Indian isolate deposited in the NCBI database. This result shows that BBTV rep gene is highly conserved among the different isolates of BBTV that infect the banana in India. Hence, an attempt was made to construct the RNAi vector for BBTV rep gene and developed method for evaluate the silencing mechanism through injection of Agrobacterium transient expression system in BBTV infected hill banana plants Virupakshi (AAB) using RNAi technology to silence and suppress the rep gene of BBTV
ICAR
 
Date 2018-07-09T10:42:39Z
2018-07-09T10:42:39Z
2017-06-01
 
Type Research Paper
 
Identifier Elayabalan, Sivalingam & Subramaniam, Sreeramanan & SELVARAJAN, RAMASAMY. (2017). Construction of BBTV rep gene RNAi Vector and Evaluate the Silencing Mechanism through Injection of Agrobacterium tumefaciens Transient Expression System in BBTV Infected Hill Banana Plants cv.Virupakshi (AAB). Indian Journal of Natural Sciences. 7. 976-997.
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http://krishi.icar.gov.in/jspui/handle/123456789/6147
 
Language English
 
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