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Detection of Specific Polypeptide(s) Synthesized during the Sequential Stages of Differentiation in Dioscorea species

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Title Detection of Specific Polypeptide(s) Synthesized during the Sequential Stages of Differentiation in Dioscorea species
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Creator Ashwani Kumar, Suresh Chand Goyal, Pooja Dhansu, Parvender Sheoran, Arvind Kumar, Ekta Verma, Jagdish Parshad and Anshuman Singh
 
Subject Dioscorea alata, Dioscorea deltoidea, callus, differentiation, polypeptides
 
Description Not Available
The present investigation was aimed to detect the specific polypeptide(s) appeared during the sequential stages of differentiation. Among different explants, only nodal explants showed good results for callusing. Depending on the fresh and dry weight, best callus growth was observed on MS medium supplemented with NAA (2.5 mg/L) in Dioscorea alata and 2, 4-D (2.0 mg/L) in D. deltoidea, respectively. This callus was used for the regeneration. Roots differentiation was observed on MS medium + NAA (2.0 mg/L) + IBA (0.5 mg/L) and shoots on MS medium + BAP (2.0 mg/L) + NAA (0.5 mg/L) in D. alata while in D. deltoidea, roots on RT medium + IAA (1.0 mg/L) and shoots on RT medium + BAP (1.0 mg/L) + NAA (0.5 mg/L). Continuous decrease was seen in the total soluble protein during the differentiation in D. alata whereas in D. deltoidea, the protein content decreased upto initiation stage. Four root specific polypeptides (MW 25.56, 24.35, 19.13 and 18.2 kDa) and three shoot specific polypeptides (MW 53.7, 25.12 and 19.13 kDa) were synthesized during the differentiation in D. alata. Similarly, two root specific (MW 33.9 and 31.69 kDa) and one shoot specific (MW 16.98 kDa) polypeptide band were appeared during differentiation in D.deltoidea.
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Date 2018-11-14T11:42:40Z
2018-11-14T11:42:40Z
2015-09-01
 
Type Research Paper
 
Identifier Not Available
1516-8913
http://krishi.icar.gov.in/Publication/handle/123456789/11308
 
Language English
 
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Publisher Not Available