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Rapid protocol for callus induction and differentiation of roots and shoots in Dioscorea alata—a medicinal plant

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Title Rapid protocol for callus induction and differentiation of roots and shoots in Dioscorea alata—a medicinal plant
 
Creator ASHWANI KUMAR, S C GOYAL, SUBHASH KAJLA and NEELAM SHARMA
 
Subject Callus induction, Differentiation, Yam
 
Description The present study was carried out to investigate the effect of different concentrations of growth regulators on in vitro
differentiation of Dioscorea through nodal explants which provide a fast, reliable and alternate method of multiplication
and facilitate ex situ conservation of this valuable plant species. Nodal explants excised from in vivo grown plants were
cultured on Murashige and Skoog (MS) medium supplemented with different combinations of plant growth substances.
The maximum (100 per cent) callus induction as well as growth was obtained on NAA (2.5 mg/l). The induced callus was
transferred onto the nutrient media supplemented with different combinations of growth regulators for the differentiation
of roots and shoots. Roots were developed on MS medium + NAA (2.0 mg/l) + IBA (0.5 mg/l), shoots on MS + BAP (2.0
mg/l) + NAA (0.5 mg/l) and roots-shoots on MS + NAA (2.5 mg/l) + 2, 4-D (2.0 mg/l) from the callus.
 
Date 2017-06-09T04:36:32Z
2017-06-09T04:36:32Z
2014-01
 
Type Research Paper
 
Identifier Kumar, A., Goyal, S. C., Kajla, S., & Sharma, N. (2014). Rapid protocol for callus induction and differentiation of roots and shoots in Dioscorea alata-a medicinal plant. Indian Journal of Agricultural Sciences, 84(1), 107-111.
http://krishi.icar.gov.in/jspui/handle/123456789/4300
 
Language English
 
Publisher ICAR