Rapid protocol for callus induction and differentiation of roots and shoots in Dioscorea alata—a medicinal plant
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Title |
Rapid protocol for callus induction and differentiation of roots and shoots in Dioscorea alata—a medicinal plant
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Creator |
ASHWANI KUMAR, S C GOYAL, SUBHASH KAJLA and NEELAM SHARMA
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Subject |
Callus induction, Differentiation, Yam
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Description |
The present study was carried out to investigate the effect of different concentrations of growth regulators on in vitro differentiation of Dioscorea through nodal explants which provide a fast, reliable and alternate method of multiplication and facilitate ex situ conservation of this valuable plant species. Nodal explants excised from in vivo grown plants were cultured on Murashige and Skoog (MS) medium supplemented with different combinations of plant growth substances. The maximum (100 per cent) callus induction as well as growth was obtained on NAA (2.5 mg/l). The induced callus was transferred onto the nutrient media supplemented with different combinations of growth regulators for the differentiation of roots and shoots. Roots were developed on MS medium + NAA (2.0 mg/l) + IBA (0.5 mg/l), shoots on MS + BAP (2.0 mg/l) + NAA (0.5 mg/l) and roots-shoots on MS + NAA (2.5 mg/l) + 2, 4-D (2.0 mg/l) from the callus. |
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Date |
2017-06-09T04:36:32Z
2017-06-09T04:36:32Z 2014-01 |
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Type |
Research Paper
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Identifier |
Kumar, A., Goyal, S. C., Kajla, S., & Sharma, N. (2014). Rapid protocol for callus induction and differentiation of roots and shoots in Dioscorea alata-a medicinal plant. Indian Journal of Agricultural Sciences, 84(1), 107-111.
http://krishi.icar.gov.in/jspui/handle/123456789/4300 |
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Language |
English
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Publisher |
ICAR
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