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Indirect ELISA using recombinant nonstructural protein 3D to detect foot and mouth disease virus infection associated antibodies

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Title Indirect ELISA using recombinant nonstructural protein 3D to detect foot and mouth disease virus infection associated antibodies
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Creator Sonalika Mahajan
Jajati Keshari Mohapatra
Laxmi Kant Pandey
Gaurav Kumar Sharma
Bramhadev Pattnaik
 
Subject FMD serosurveillance3DNonstructural protein ELISA
 
Description Not Available
Foot-and-mouth disease (FMD) is a transboundary animal disease caused by foot-and-mouth disease virus. In India, systematic preventive vaccination using inactivated trivalent (O, A and Asia 1) vaccine is the strategy being adopted to control FMD. The use of non-structural protein (NSP)-contaminated inactivated vaccine raises concerns over differentiation of infected and vaccinated animals (DIVA) by NSP based immunoassays. However, 2C being a membrane associated protein usually remain absent in vaccine formulations and thus, anti-2C response is one of the most reliable indicator of the FMDV infection. In this study, 34 amino acids from N-terminus of 2C protein were removed to eliminate membrane-binding amphipathic helicase activity for the expression of recombinant protein in soluble form. Truncated 2C (2Ct) was utilized for development of an indirect ELISA (I-ELISA) for bovine and the developed 2Ct I-ELISA was validated using a panel constituting of serum of naïve, vaccinated and infected animals. The assay was compared with the in-house r3AB3 I-ELISA and the overall concordance was 85.31%. The diagnostic sensitivity and specificity of the 2Ct I-ELISA were 92.9% and 94.0%, respectively. The apparent prevalence of anti-2C antibodies for random bovine samples tested by the developed assay was 23.7%. The developed ELISA will help in augmenting the sensitivity of detection if used in combination with r3AB3 I-ELISA for sero-surveillance.
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Date 2021-08-01T12:06:05Z
2021-08-01T12:06:05Z
2015-01
 
Type Research Paper
 
Identifier Mahajan S, Mohapatra JK, Pandey LK, Sharma GK, Pattnaik B. Indirect ELISA using recombinant nonstructural protein 3D to detect foot and mouth disease virus infection associated antibodies. Biologicals. 2015 Jan;43(1):47-54. doi: 10.1016/j.biologicals.2014.10.002. Epub 2014 Nov 12. PMID: 25458472.
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http://krishi.icar.gov.in/jspui/handle/123456789/51835
 
Language English
 
Relation Not Available;
 
Publisher Elsevier