Indirect ELISA using recombinant nonstructural protein 3D to detect foot and mouth disease virus infection associated antibodies
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Title |
Indirect ELISA using recombinant nonstructural protein 3D to detect foot and mouth disease virus infection associated antibodies
Not Available |
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Creator |
Sonalika Mahajan
Jajati Keshari Mohapatra Laxmi Kant Pandey Gaurav Kumar Sharma Bramhadev Pattnaik |
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Subject |
FMD serosurveillance3DNonstructural protein ELISA
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Description |
Not Available
Foot-and-mouth disease (FMD) is a transboundary animal disease caused by foot-and-mouth disease virus. In India, systematic preventive vaccination using inactivated trivalent (O, A and Asia 1) vaccine is the strategy being adopted to control FMD. The use of non-structural protein (NSP)-contaminated inactivated vaccine raises concerns over differentiation of infected and vaccinated animals (DIVA) by NSP based immunoassays. However, 2C being a membrane associated protein usually remain absent in vaccine formulations and thus, anti-2C response is one of the most reliable indicator of the FMDV infection. In this study, 34 amino acids from N-terminus of 2C protein were removed to eliminate membrane-binding amphipathic helicase activity for the expression of recombinant protein in soluble form. Truncated 2C (2Ct) was utilized for development of an indirect ELISA (I-ELISA) for bovine and the developed 2Ct I-ELISA was validated using a panel constituting of serum of naïve, vaccinated and infected animals. The assay was compared with the in-house r3AB3 I-ELISA and the overall concordance was 85.31%. The diagnostic sensitivity and specificity of the 2Ct I-ELISA were 92.9% and 94.0%, respectively. The apparent prevalence of anti-2C antibodies for random bovine samples tested by the developed assay was 23.7%. The developed ELISA will help in augmenting the sensitivity of detection if used in combination with r3AB3 I-ELISA for sero-surveillance. Not Available |
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Date |
2021-08-01T12:06:05Z
2021-08-01T12:06:05Z 2015-01 |
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Type |
Research Paper
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Identifier |
Mahajan S, Mohapatra JK, Pandey LK, Sharma GK, Pattnaik B. Indirect ELISA using recombinant nonstructural protein 3D to detect foot and mouth disease virus infection associated antibodies. Biologicals. 2015 Jan;43(1):47-54. doi: 10.1016/j.biologicals.2014.10.002. Epub 2014 Nov 12. PMID: 25458472.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/51835 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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