Record Details

Multiresidue Analysis of Multiclass Plant Growth Regulators in Grapes by Liquid Chromatography/Tandem Mass Spectrometry

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Multiresidue Analysis of Multiclass Plant Growth Regulators in Grapes by Liquid Chromatography/Tandem Mass Spectrometry
 
Creator Dasharath P. Oulkar, Kaushik Banerjee,1 Manoj S. Ghaste, and Sahadeo D. Ramteke
 
Subject PGRs, GA3, Residue
 
Description Not Available
A selective and rapid multiresidue analysis method is presented for simultaneous estimation of 12 plant growth regulators (PGRs), namely, auxins (indol-3-acetic acid, indol-3-butyric acid, and naphthyl acetic acid), cytokinins (kinetin, zeatin, and 6-benzyladenine), gibberellic acid (GA3), abscisic acid, and synthetic compounds, namely, forchlorfenuron, paclobutrazole, isoprothiolane, and 2,4-dichlorophenoxy acetic acid (2,4-D) in bud sprouts and grape berries at the development stages of 2–3 and 6–8 mm diameters, which are the critical phases when exogenous application of PGRs may be necessary to achieve desired grape quality and yield. The sample preparation method involved extraction of plant material with acidified methanol (50%) by homogenization for 2 min at 15 000 rpm. The pH of the extract was enhanced up to 6 by adding ammonium acetate, followed by homogenization and centrifugation. The supernatant extract was cleaned by SPE on an Oasis HLB cartridge (200 mg, 6 cc). The final extract was measured directly by LC/MS/MS with electrospray ionization in positive mode, except for 2,4-D, GA3, and abscisic acid extracts, which required analysis in negative mode. Quantification by multiple reaction monitoring (MRM) was supported with full-scan mass spectrometric confirmation using “information-dependent acquisition” triggered with MRM to “enhanced product ionization” mode of the hybrid quadrupole-ion trap mass analyzer. The LOQ of the test analytes varied between 1 and 10 ng/g with associated recoveries of 80–120% and precision RSD
Not Available
 
Date 2021-07-08T05:49:14Z
2021-07-08T05:49:14Z
2010-08-31
 
Type Article
 
Identifier Dasharath P. Oulkar, Kaushik Banerjee, Manoj S. Ghaste, and Sahadeo D. Ramteke (2011). Multiresidue Analysis of Multiclass Plant Growth Regulators in Grapes by Liquid Chromatography/Tandem Mass Spectrometry. Journal of AOAC Internatio nal Vol. 94, No. 3 Pp 968-977
1060-3271
http://krishi.icar.gov.in/jspui/handle/123456789/47556
 
Language English
 
Relation Not Available;
 
Publisher Not Available