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Amperometric Biosensor Approaches for Quantification of Indole 3-Acetic Acid in Plant Stress Responses

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Title Amperometric Biosensor Approaches for Quantification of Indole 3-Acetic Acid in Plant Stress Responses
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Creator Krishnamurthy K. S., Diggs, A., Porterfield, D.M.
 
Subject Amperometric biosensor, ELISA, HPLC, indole acetic acid, nanomaterial, plant tissue, silanization
 
Description Not Available
Amperometric biosensors are known to be sensitive, reliable, and inexpensive instruments
for biomolecular detection. Recently, self-referencing amperometric biosensors
have been utilized to quantify the endogenous apoplastic flux of plant hormone indole
3-acetic acid (IAA) in vivo. There is still a significant need for sampling and testing
methods to measure IAA concentrations in whole tissue samples. In the present
study we used nanomaterial-modified platinum microelectrodes for the detection of
IAA extracted from whole plant tissues. The key to the use of the nanomaterials was
to enhance the surface area and thus the limit of detection for IAA. The nanoscale
electrochemical interface was modified by the application of a layer of platinum black,
followed by silanization overnight and a coating of multiwalled carbon nanotubes.
Electroanalytical characterization of sensor performance was evaluated using both IAA
standards and IAA extracted from corn plant samples, using a three-electrode scheme
(reference, sensing, and auxillary). We compared tissue concentrations in water- and
salt-stressed corn seedlings and compared these results to values measured using established
enzyme-linked immunosorbent assay (ELISA) protocols.We found that the values
obtained from both methods were comparable. The data obtained from the IAA sensor
suggested that the electroanalytical biosensor approach was slightly more reliable and
sensitive. Our results demonstrate a novel nanomaterial biosensor approach for IAA
quantification in plant tissue extracts. The results of the stress study clearly indicated
that root and shoot elongation and growth had significant positive correlation with IAA
content in the raw plant tissue extracts. Water and salt stress both reduced root and
shoot growth, which may be due to overaccumulation of IAA, resulting in inhibition
of elongation. In comparison to established methods, such as high-performance liquid
chromatography (HPLC) or ELISA, our approach is simple and inexpensive. Unlike
HPLC, our approach does not require any elaborate sample purification, nor does it require antibody development as needed for ELISA. In summary, this electroanalytical
biosensor method can be effectively utilized for simple, cheap, and reliable detection of
IAA extracted from plant samples.
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Date 2021-07-27T06:29:01Z
2021-07-27T06:29:01Z
2013-06-06
 
Type Research Paper
 
Identifier Krishnamurthy K. S., A. Diggs & D. M. Porterfield 2013 Amperometric Biosensor Approaches for Quantification of Indole 3-Acetic Acid in Plant Stress Responses, Communications in Soil Science and Plant Analysis, 44 (11) : 1749-1763 DOI: 10.1080/00103624.2013.783920
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http://krishi.icar.gov.in/jspui/handle/123456789/50235
 
Language English
 
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Publisher Not Available