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Identification and characterization of cardamom vein clearing virus, a novel aphid-transmitted nucleorhabdovirus

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Title Identification and characterization of cardamom vein clearing virus, a novel aphid-transmitted nucleorhabdovirus
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Creator A. I.Bhat
N.S.Pamitha
K.P. Naveen
C.N. Biju
 
Subject aphid transmission, small RNA sequencing, RNA sequencing, RT-PCR, real-time RT-PCR, detection
 
Description Not Available
The vein clearing (kokke kandu) disease suspected to be caused by an unknown virus is an important production constraint of cardamom in India. In the present study the causal virus was transmitted from infected to healthy cardamom plants through the aphid, Pentalonia caladii. Small RNA (sRNA) and RNA sequencing (RNA-seq) of the aphid-inoculated plant showed several nucleorhabdovirus-like contigs. The sRNA and RNA-seq results were verified through reverse transcription polymerase chain reaction (RT-PCR) using total RNA from infected plant and primers designed fromthe contigs. The cloning and sequencing of RT-PCR products resulted in a sequence of 13392 bases that showed similarities to nucleorhabdoviruses. Thesequenced region contained allsix open reading frames of the virusin the order 3'-N-P-P3-M-G-L-5'and showed nucleotide sequence identities ranging from 37-55% with nucleorhabdoviruses indicating its distinct nature for which we propose the name, cardamom vein clearing virus.A reliable RT-PCR and SYBR Green-based real-time RT-PCR assays were developed for the detection of the virus that willaid in the identification and propagation of virus-free cardamom plants.
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Date 2021-07-28T05:17:37Z
2021-07-28T05:17:37Z
2020-02-15
 
Type Research Paper
 
Identifier Bhat A I, Pamitha NS, Naveen KP and Biju CN. 2020. Identification and characterization of cardamom vein clearing virus, a novel aphid-transmitted nucleorhabdovirus. European Journal of Plant Pathology. 156:1053–1062. https://doi.org/10.1007/s10658-020-01958-2
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http://krishi.icar.gov.in/jspui/handle/123456789/50422
 
Language English
 
Relation Not Available;
 
Publisher Springer