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Development of diagnostic assays for rapid and sensitive detection of Phytophthora infecting major spices and plantation crops

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Title Development of diagnostic assays for rapid and sensitive detection of Phytophthora infecting major spices and plantation crops
Not Available
 
Creator R.T.P.Pandian
A. I. Bhat
C. N. Biju
S. Sasi
 
Subject : black pepper, loop-mediated isothermal amplification, Phytophthora, polymerase chain reaction, real-time LAMP, real-time PCR
 
Description Not Available
Phytophthora, the ubiquitous stramenopile phytopathogen is a major threat to several economically
important horticultural crops including spices and plantation crops. Trans-seasonal survival of
Phytophthora in plant debris and soil continuum has considerable epidemiological significance as the
quiescent propagules often serve as primary foci of infection with inherent potential to trigger
epiphytotics in the succeeding season favoured by conducive environmental conditions. Hence,
early and rapid detection of over summering propagules is highly imperative to manage Phytophthorainduced diseases efficiently and economically. Twelve isolates representing different species of
Phytophthora (P. capsici, P. tropicalis, P. palmivora. P. citrophthora and P. meadii) representing hosts such
as black pepper, cardamom, nutmeg, coconut, arecanut and cocoa were used to develop nucleic
acid-based diagnostic tools viz., polymerase chain reaction (PCR), real-time PCR, loop-mediated
isothermal amplification (LAMP) and real-time LAMP. Phytophthora genus-specific primers were
designed from the conserved region of nuclear ribosomal DNA. Each of the assays was specific and
detected different species of Phytophthora and not other pathogens (Rhizoctonia solani, Pythium vexans,
Fusarium oxysporum and Colletotrichum gloeosporioides) and plant samples. Sensitivity assays indicated
that, real-time PCR detected Phytophthora upto 1.3 fg, followed by LAMP (13 fg) and PCR (13 pg).
Not Available
 
Date 2021-07-28T08:12:53Z
2021-07-28T08:12:53Z
2018-10-18
 
Type Research Paper
 
Identifier Pandian, RTP, Bhat,AI, Biju CN, and Sasi S. 2018. Development of diagnostic assays for rapid and sensitive detection of Phytophthora infecting major spices and plantation crops. J Spices and Aromatic Crops 27: 119 – 130
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/50638
 
Language English
 
Relation Not Available;
 
Publisher Indian Society for Spices