Record Details

Rapid detection of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) by loop-mediated isothermal amplification (LAMP)

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Rapid detection of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) by loop-mediated isothermal amplification (LAMP)
Not Available
 
Creator Not Available
A.I.Bhat
A.Siljo
K.P.Deeshma
 
Subject cucumber mosaic virus, piper yellow mottle virus, isothermal detection, sensitivity, PCR
 
Description Not Available
The loop-mediated isothermal amplification (LAMP) assay for Piper yellow mottle virus and the reverse
transcription (RT) LAMP assay for Cucumber mosaic virus each consisted of a set of five primers designed
against the conserved sequences in the viral genome. Both RNA and DNA isolated from black pepper
were used as a template for the assay. The results were assessed visually by checking turbidity, green
fluorescence and pellet formation in the reaction tube and also by gel electrophoresis. The assay success fully detected both viruses in infected plants whereas no cross-reactions were recorded with healthy
plants. Optimum conditions for successful amplification were determined in terms of the concentrations
of magnesium sulphate and betaine, temperature, and duration. The detection limit for both LAMP and
RT-LAMP was up to 100 times that for conventional PCR and up to one-hundredth of that for real-time
PCR. The optimal conditions arrived at were validated by testing field samples of infected vines of three
species from different regions
Not Available
 
Date 2021-07-28T11:14:21Z
2021-07-28T11:14:21Z
2013-06-19
 
Type Research Paper
 
Identifier Bhat, A.I., Siljo, A. and Deeshma, K.P. 2013. Rapid detection of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) by loop-mediated isothermal amplification (LAMP). Journal of Virological Methods 193: 190-196.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/50827
 
Language English
 
Relation Not Available;
 
Publisher Elseveir