Record Details

Occurrence of Banana bract mosaic virus on cardamom

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Occurrence of Banana bract mosaic virus on cardamom
Not Available
 
Creator Not Available
A.Siljo
A. I. Bhat
C.N. Biju
M.N.Venugopal
 
Subject Cardamom mosaic virus. Chlorotic streak . Coat protein . Detection . Elettaria cardamomum . India . RT-PC
 
Description Not Available
During a survey for mosaic disease caused by Cardamom mosaic virus (CdMV) in cardamom plantations, a new kind of viral disease showing chlorotic streak on veins was observed in some plantations. Based on the type of symptom, the disease was named ‘chlorotic streak’. In a survey of 77 cardamom plantations at 49 locations in the states of Kerala, Karnataka and Tamil Nadu in India, incidence of the disease ranged from zero to 15%. The plants infected with chlorotic streak tested negative in reverse transcription polymerase chain reaction (RT-PCR) for CdMV. Leaf dip electron microscopy of these samples showed the presence of
flexuous virions resembling Potyvirus. Total RNA extracted from these plants when subjected to RT-PCR
using primers targeted to conserved region in Potyvirus resulted in an amplicon size of ~700 bp. Sequencing and BLAST analysis of this amplicon showed Banana bract mosaic virus (BBrMV) as the
closest virus. Using primers specific for BBrMV, the coat protein gene from five different geographical
isolates of the virus was cloned and sequenced. Sequence analysis of the coat protein gene showed
an identity of >94% with BBrMV isolates while identity with other distinct potyvirus species was

Not Available
 
Date 2021-07-28T11:14:32Z
2021-07-28T11:14:32Z
2011-09-02
 
Type Research Paper
 
Identifier Siljo, A., Bhat, A.I., Biju, C.N. and Venugopal, M.N. 2012. Occurrence of Banana bract mosaic virus on cardamom. Phytoparasitica. 40:77-85.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/50828
 
Language English
 
Relation Not Available;
 
Publisher Springer