Occurrence of Banana bract mosaic virus on cardamom
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Title |
Occurrence of Banana bract mosaic virus on cardamom
Not Available |
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Creator |
Not Available
A.Siljo A. I. Bhat C.N. Biju M.N.Venugopal |
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Subject |
Cardamom mosaic virus. Chlorotic streak . Coat protein . Detection . Elettaria cardamomum . India . RT-PC
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Description |
Not Available
During a survey for mosaic disease caused by Cardamom mosaic virus (CdMV) in cardamom plantations, a new kind of viral disease showing chlorotic streak on veins was observed in some plantations. Based on the type of symptom, the disease was named ‘chlorotic streak’. In a survey of 77 cardamom plantations at 49 locations in the states of Kerala, Karnataka and Tamil Nadu in India, incidence of the disease ranged from zero to 15%. The plants infected with chlorotic streak tested negative in reverse transcription polymerase chain reaction (RT-PCR) for CdMV. Leaf dip electron microscopy of these samples showed the presence of flexuous virions resembling Potyvirus. Total RNA extracted from these plants when subjected to RT-PCR using primers targeted to conserved region in Potyvirus resulted in an amplicon size of ~700 bp. Sequencing and BLAST analysis of this amplicon showed Banana bract mosaic virus (BBrMV) as the closest virus. Using primers specific for BBrMV, the coat protein gene from five different geographical isolates of the virus was cloned and sequenced. Sequence analysis of the coat protein gene showed an identity of >94% with BBrMV isolates while identity with other distinct potyvirus species was Not Available |
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Date |
2021-07-28T11:14:32Z
2021-07-28T11:14:32Z 2011-09-02 |
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Type |
Research Paper
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Identifier |
Siljo, A., Bhat, A.I., Biju, C.N. and Venugopal, M.N. 2012. Occurrence of Banana bract mosaic virus on cardamom. Phytoparasitica. 40:77-85.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/50828 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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