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Transcriptome Analysis of Cocos nucifera L. Seedlings Having Contrasting Water-Use Efficiency (WUE) under Water-Deficit Stress: Molecular Insights and Genetic Markers for Drought Tolerance

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Title Transcriptome Analysis of Cocos nucifera L. Seedlings Having Contrasting Water-Use Efficiency (WUE) under Water-Deficit Stress: Molecular Insights and Genetic Markers for Drought Tolerance
Not Available
 
Creator S. V. Ramesh , K. B. Hebbar *, M. K. Rajesh, P. Abhin Sukumar, K. P. Ganga Raj and Athul Bobby
 
Subject coconut seedlings; climate-smart; drought adaptation; genic markers; RNA-seq
 
Description Not Available
Perennials utilize complex adaptive strategies and molecular mechanisms to cope with
water-deficit conditions. Hence, to gain molecular insights regarding the water-deficit stress, twoyear-
old coconut seedlings of the varieties Kalpa Sree and Kalpatharu were subjected to soil waterdeficit
regimes (25% of available of soil moisture and control). Biochemical, physiological and
growth parameters underlying water-deficit stress revealed the differential enzymatic antioxidants,
lipid peroxidation status and water use efficiency trait between the genotypes
investigated. The whole plant water use efficiency at control condition was significantly low in
Kalpatharu (4.06) compared to Kalpa Sree (4.74). Nevertheless, under severe stress [25% ASM]
Kalpatharu exhibited highest WUE (5.68) as against dwarf variety Kalpa Sree (3.84). Furthermore,
the leaf transcriptome profiles of the control and water-deficit stressed seedlings were examined
utilizing paired-end RNA-Seq. In total, ~7300 differentially expressed genes have been identified
between the seedlings under water-deficit stress and control. Analysis of control and stressed
Kalpasree leaf transcriptome showed significant upregulation of PHLOEM PROTEIN 2-LIKE A1-
like, WRKY transcription factor 40 isoform X1 and downregulation of glycerol-3-phosphate
acyltransferase 3 transcripts. On the other hand, upregulation of transcripts encoding polyamine
oxidase, arabinose 5-phosphate isomerase among others and downregulation of aquaporin PIP1-2
transcript was documented in Kalpatharu leaves. Besides, long non-coding RNA and genic SSRs
were also identified from the transcriptome data to further enrich the genomic resources of coconut
palm which would pave way for its utilization in developing climate-smart coconut crop. The
implication of this study in molecular dissection of the adaptive response of coconut to the soilwater
deficit is also discussed.
Not Available
 
Date 2021-07-30T04:54:50Z
2021-07-30T04:54:50Z
2020-12-02
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/51356
 
Language English
 
Relation Not Available;
 
Publisher MDPI