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Simultaneous elimination of Cucumber mosaic virus and Cymbidium mosaic virus infecting Vanilla planifolia through meristem culture

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Title Simultaneous elimination of Cucumber mosaic virus and Cymbidium mosaic virus infecting Vanilla planifolia through meristem culture
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Creator S. T. Retheesh
A.I. Bhat
 
Subject Cucumber mosaic virus, Cymbidium mosaic virus, Meristem culture, RT-PCR, Vanilla, Virus elimination
 
Description Not Available
Vanilla (Vanilla planifolia G. Jackson), a perennial fleshy vine infected by Cucumber mosaic virus (CMV) and Cymbidium mosaic virus (CymMV) was freed from infection through meristem culture. Apical meristem measuring 0.1 to 0.25 mm were isolated and cultured in Murashige and Skoog (MS) medium supplemented with 0.45 µM thidiazuron for 40-45 days to initiate the growth. Following enlargement of meristem, it was transferred to MS medium supplemented with 4.43 µM 6-benzyl aminopurine (BAP) and 2.68 µM α-naphthalene acetic acid (NAA) for regeneration. The regenerated plantlets were hardened in insect-free glasshouse and confirmed for the elimination of viruses using reverse transcription PCR (RT-PCR) with virus specific primer pairs. The frequency of CMV elimination was 79.4% while that of CymMV was 82.4% when tested individually. We obtained a frequency of 75% simultaneous elimination of both viruses. This is the first report of meristem culture and virus elimination in vanilla.
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Date 2021-07-29T05:31:44Z
2021-07-29T05:31:44Z
2010-05-01
 
Type Research Paper
 
Identifier Retheesh, S.T. and Bhat, A.I. 2010. Simultaneous elimination of Cucumber mosaic virus and Cymbidium mosaic virus infecting Vanilla planifolia through meristem culture. Crop Protection, 29: 1214-1217. doi:10.1016/j.cropro.2010.05.017
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http://krishi.icar.gov.in/jspui/handle/123456789/50953
 
Language English
 
Relation Not Available;
 
Publisher Springer