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Coat protein gene sequence studies suggest that Cucumber mosaic virus infecting paprika (Capsicum annum L.) in India belongs to subgroup IB

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Title Coat protein gene sequence studies suggest that Cucumber mosaic virus infecting paprika (Capsicum annum L.) in India belongs to subgroup IB
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Creator V. Bhadramurthy
Anju George
A.I. Bhat
K.N.Shiva
 
Subject Paprika, Capsicum annuum, Cucumber mosaic virus, coat protein gene, sequence analyses, phylogenetic relationships
 
Description Not Available
Cucumber mosaic virus (CMV) causing mosaic, leaf distortion and stunting on paprika (Capsicum annuum
L.) in India was detected by direct antigen-coated enyme linked immunosorbent assay (DAC-ELISA)
and reverse transcription polymerase chain reaction (RT-PCR). The coat protein (CP) gene of the virus
was amplified, cloned and sequenced. Sequenced region contained a single open reading frame of 657
nucleotides potentially coding for 218 amino acids. Amino acid and nucleotide sequence analyses of
CMV CP with members of subgroup IA, IB and II revealed that CMV infecting paprika showed the
greatest identity with members of subgroup IB. The identity of amino acid and nucleotide sequences
ranged from 95% and 91 –92% with members of subgroup IA, 94 – 99% and 93 –97% with subgroup IB
isolates and 78 –82% and 75 –76% with members of subgroup II. The results were also confirmed
through phylogram based on multiple sequence alignments of coat protein gene. This is the first report
on molecular characterization of CMV infecting paprika in India.
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Date 2021-07-29T05:32:21Z
2021-07-29T05:32:21Z
2009-09-01
 
Type Research Paper
 
Identifier Bhadramurthy, V., Anju George, Bhat, A.I. and Shiva, K.N 2009. Coat protein gene sequence studies suggest that Cucumber mosaic virus infecting paprika (Capsicum annum L.) in India belongs to subgroup IB. Archives of Plant Pathology and Plant Protection, 42: 857-863
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http://krishi.icar.gov.in/jspui/handle/123456789/50956
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis