Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress
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Title |
Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress
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Creator |
Johnson George, K., Neema Malik, Vijesh Kumar, I.P., Krishnamurthy, K.S.
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Subject |
Black pepper, Relative water content, Drought, Dehydrin, Osmotin, Aquaporin, Gene expression, Real time PCR
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Description |
Not Available
Drought or water deficit stress is one of the main environmental stresses affecting plants, resulting in reduced productivity and crop loss. Black pepper, a major spice cultivated across the globe, is drought sensitive and water stress often results in plant death. The present study compared the difference in physiological parameters: rel- ative water content (RWC) and cell membrane leakage, and also analyzed the differential expression of 11 drought responsive genes in drought tolerant and drought sensitive black pepper genotypes. Tolerant black pepper genotype exhibited significantly higher RWC and lower cell mem- brane leakage 10 days after stress induction than the sen- sitive genotype. The relative expressions of the 11 selected drought responsive genes were normalized against ubiq- uitin and RNA-binding protein which was identified as the most stable reference genes in black pepper under the present experimental condition using the RefFinder soft- ware. Dehydrin showed the highest transcript accumulation in both the black pepper genotypes under drought stress condition and the relative expression of the gene was higher in the tolerant genotype compared to the suscepti- ble. Similar pattern of higher relative expression was also observed in the stress responsive gene, osmotin. The membrane protein aquaporin and the transcription factorbZIP were relatively down-regulated in the tolerant geno- type. The differential expression of these important drought responsive genes in tolerant genotype of black pepper indicates its further usefulness in developing varieties with improved water stress tolerance. Not Available |
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Date |
2021-07-29T09:13:16Z
2021-07-29T09:13:16Z 2017-03-22 |
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Type |
Research Paper
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Identifier |
Johnson George, K., Neema Malik, Vijesh Kumar, I.P. and Krishnamurthy, K.S. 2017 Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress. Acta Physiologiae Plantarum 39: 104
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/51051 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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