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Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress

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Title Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress
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Creator Johnson George, K., Neema Malik, Vijesh Kumar, I.P., Krishnamurthy, K.S.
 
Subject Black pepper, Relative water content, Drought, Dehydrin, Osmotin, Aquaporin, Gene expression, Real time PCR
 
Description Not Available
Drought or water deficit stress is one of the main
environmental stresses affecting plants, resulting in
reduced productivity and crop loss. Black pepper, a major
spice cultivated across the globe, is drought sensitive and
water stress often results in plant death. The present study
compared the difference in physiological parameters: rel-
ative water content (RWC) and cell membrane leakage,
and also analyzed the differential expression of 11 drought
responsive genes in drought tolerant and drought sensitive
black pepper genotypes. Tolerant black pepper genotype
exhibited significantly higher RWC and lower cell mem-
brane leakage 10 days after stress induction than the sen-
sitive genotype. The relative expressions of the 11 selected
drought responsive genes were normalized against ubiq-
uitin and RNA-binding protein which was identified as the
most stable reference genes in black pepper under the
present experimental condition using the RefFinder soft-
ware. Dehydrin showed the highest transcript accumulation
in both the black pepper genotypes under drought stress
condition and the relative expression of the gene was
higher in the tolerant genotype compared to the suscepti-
ble. Similar pattern of higher relative expression was also
observed in the stress responsive gene, osmotin. The
membrane protein aquaporin and the transcription factorbZIP were relatively down-regulated in the tolerant geno-
type. The differential expression of these important drought
responsive genes in tolerant genotype of black pepper
indicates its further usefulness in developing varieties with
improved water stress tolerance.
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Date 2021-07-29T09:13:16Z
2021-07-29T09:13:16Z
2017-03-22
 
Type Research Paper
 
Identifier Johnson George, K., Neema Malik, Vijesh Kumar, I.P. and Krishnamurthy, K.S. 2017 Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress. Acta Physiologiae Plantarum 39: 104
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http://krishi.icar.gov.in/jspui/handle/123456789/51051
 
Language English
 
Relation Not Available;
 
Publisher Not Available