Noncoding RNA-Based Genetically Modified Crops: Concepts and Challenges
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Title |
Noncoding RNA-Based Genetically Modified Crops: Concepts and Challenges
Not Available |
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Creator |
S.V. Ramesh, and Shelly Praveen
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Subject |
non-coding RNAs, Genetic modification, ERA
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Description |
Not Available
In addition to expression of foreign proteins, crop genetic engineering based on small noncoding RNAs (sncRNAs) form an integral component of crop improvement programs. ncRNA-based genetic manipulation is a potent gene silencing tool whenever repression of gene(s) is desired. Gene silencing is achieved mainly by deploying sncRNAs like small interfering RNAs (siRNAs) and artificial miRNAs (amiRNAs). It also offers an opportunity for crop metabolic engineering employing synthetic trans-acting siRNAs (syn-tasiRNAs). Since ncRNA-based genetically modified (GM) crops differ significantly from first-generation protein-based transgenics, there are many apprehensions with regard to off-target and nontarget effects as well as mutational effects on the efficacy of ncRNA silencing, etc. Despite the apprehensions associated with ncRNA-based GM crops, the technology has been successfully employed for crop genetic engineering in 12 crops, incorporating traits ranging from resistance to pathogen, allergen removal, nutritional improvement, etc. The chapter discusses other pertinent issues like persistence of ncRNAs, food and feed safety, and future prospects of ncRNA-based GM crops. Not Available |
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Date |
2021-07-30T04:50:43Z
2021-07-30T04:50:43Z 2015-10-01 |
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Type |
Book chapter
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/51335 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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