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Apocarotenoid gene expression in saffron (Crocus sativus L.).

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Title Apocarotenoid gene expression in saffron (Crocus sativus L.).
Not Available
 
Creator J I MIR
N AHMED
M H KHAN
T A MOKHDOMI
 
Subject Crocus sativus
CsZCD CsLYC
RT-PCR RNA
isolation
apocarotenoid
 
Description Not Available
Saffron (Crocus sativus L.) is a triploid sterile plant characterized by its long red stigmas, which
produce and store its chief carotenoid derivatives-safranal, crocin and picrocrocin. Saffron selections
of Kashmir are heterogeneous for floral characteristics which are mainly attributed to the environmental
factors, though genetic factors may have role with regard to its differential characteristics across
various selections found in the area. Identification of high yielding selections using the existing gene
pool of saffron may help in improving the productivity of this crop. The present study was conducted at
Biotechnology Lab, Central Institute of Temperate Horticulture, Srinagar, India during 2012-2013 to find
out the apocarotenoid gene expression in thirty-one morphogically distinct clones of saffron.
Comparative apocarotenoid gene expression through Real Time PCR analysis revealed a significant
variation in Zeaxanthin cleavage dioxygenase (CsZCD) and lycopene-β-cyclase (CsLYC) genes between
the most divergent selections (CITH-S-107 and PAM-S-116) indicating a possible role of these genes for
regulating the apocarotenoid production in stigma. Significant variation was observed with respect to
stigma length (2.86 to 4.84 cm) across thirty-one selected saffron clones. Although some clones
showed variation with respect to stigma number, in addition to normal trifid stigmas, some clones
produce tetrafid stigmas as well but this character was not heritable. Our study provides sufficient
knowledge to identify clones with better stigma characteristics and higher apocarotenoid biosynthetic
potential for further crop improvement programs
Not Available
 
Date 2021-08-23T08:42:58Z
2021-08-23T08:42:58Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
1992-2248
http://krishi.icar.gov.in/jspui/handle/123456789/59172
 
Language English
 
Relation Not Available;
 
Publisher Not Available