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A modified semi-selective medium for isolation and enumeration of Pochonia chlamydosporia (Goddard) Zare & W. Gams.

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Title A modified semi-selective medium for isolation and enumeration of Pochonia chlamydosporia (Goddard) Zare & W. Gams.
A semi-selective medium for Pochonia chlamydosporia
 
Creator M K Rincy, R Praveena, S J Eapen
 
Subject biological control, Pochonia chlamydosporia, quantification, semi-selectie medium
 
Description Not Available
Pochonia chlamydosporia, is one of the most promising biological control agents for managing phytoparasitic nematodes. Isolation and enumeration of viable colonies of P. chlamydosporia from soil and other substrates without contamination is a major limitation, when commonly available nutrient media are used. Development of a suitable selective/semi-selective media by incorporating one or more inhibitors of microbial growth can facilitate isolation of the fungus. In vitro studies were carried out to test the compatibility of commonly used pesticides, namely, metalaxyl, metalaxyl-mancozeb, carbendazim, copper oxychloride, and chlorpyrifos with P. chlamydosporia. The fungus showed relatively high tolerance to higher doses of metalaxyl and carbendazim and was used in the modified medium for better suppression of other soil borne fungi. In the present study, Kerry’s semi-selective medium was modified and evaluated by counting the viable fungal propagules in different substrates (rice, farmyard manure, maize, rice bran, barley, and sorghum) and soil artificially inoculated with the fungus. The results showed that the modified Kerry’s semi-selective medium can effectively be used for isolation and quantification of P. chlamydosporia in routine studies.
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Date 2021-08-06T15:27:47Z
2021-08-06T15:27:47Z
2021-07-12
 
Type Research Paper
 
Identifier Rincy, M. K., Praveena, R., & Eapen, S. J. (2021). A modified semi-selective medium for isolation and enumeration of Pochonia chlamydosporia (Goddard) Zare & W. Gams: A semi-selective medium for Pochonia chlamydosporia. Journal of Spices and Aromatic Crops, 30(1), 117–125. https://doi.org/10.25081/josac.2021.v30.i1.6923
0971-3328
http://krishi.icar.gov.in/jspui/handle/123456789/53493
 
Language English
 
Relation Not Available;
 
Publisher Indian Society for Spices