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Polyamine mediated genotype independent somatic embryogenesis in papaya (Carica papaya.)

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Title Polyamine mediated genotype independent somatic embryogenesis in papaya (Carica papaya.)
Not Available
 
Creator Kumari, S., Trivedi, M., Shukla, N. and Mishra, M.
 
Subject Genotype, Carica papaya L., polyamine, somatic embryogenesis, PEG
 
Description Not Available
A highly repeatable genotype independent, two-steps somatic embryogenesis protocol was developed. Immature zygotic
embryos excised from 90 to 120 days old fruit of Pusa Delicious, CO 7 and Red Lady induced somatic embryos in half strength
MS medium fortified with 10 mgl
-1
2,4-D and polyamine spermidine at 100 µM. Embryos were matured with 45 mgl
-1
polyethylene
glycol (PEG) and germinated on hormone free medium. Embryogenesis frequency among different cultivars ranged from 5.7
to 72 per cent. Rooted plantlets were successfully acclimatized in autoclaved coco peat fortified with MS salt mixture under
shade net condition. The plants were established successfully in field.
Not Available
 
Date 2021-08-12T09:51:06Z
2021-08-12T09:51:06Z
2018-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/55383
 
Language English
 
Relation Not Available;
 
Publisher Not Available