Record Details

Interplay of genes in plant–pathogen interactions: In planta expression and docking studies of a beta 1,3 glucanase gene from Piper colubrinum and a glucanase inhibitor gene from Phytophthora capsici.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Interplay of genes in plant–pathogen interactions: In planta expression and docking studies of a beta 1,3 glucanase gene from Piper colubrinum and a glucanase inhibitor gene from Phytophthora capsici.
Not Available
 
Creator Johnson George K, Rosana Babu O, Vijesh Kumar I P, Eapen S J and Anandaraj M
 
Subject Piper colubrinum, Phytophthora capsici, Black pepper, Molecular docking, qRT-PCR
 
Description Not Available
Oomycete pathogen, Phytophthora capsici is devastating for black pepper (Piper nigrum L.) and causes
foot rot disease at all stages of plant growth. Phytophthora secretes a glucanase inhibitor protein (GIP), which is capable of inhibiting defence proteins like endoglucanases. In this particular study Quantitative PCR analysis, molecular docking studies and analysis of sequences of Glucanase inhibitor protein and beta-1,3 glucanse genes were done mainly depending on the data derived from Phytophthora capsici whole genome sequencing and Piper colubrinum RNA-sequencing (RNA-Seq). Amino acid sequence length of GIP gene from P. capsici was about 353 amino acids and that of glucanase pcEGase gene from P. colubrinum was about 312 amino acids. GIP gene from P. capsici showed high level of expression at early hours of the inoculation time period and pcEGase gene showed high level of expression at 16 hpi. High level of expression of pcEGase gene at 16 hpi is an indication that the GIP gene is successfully inhibited by the glucanase protein from the
plant. Moreover in silico studies gave some hint on the importance of certain sites on the surfaces of both interacting proteins that might be having a role in binding of the two proteins and subsequent reactions thereof. Insilico analysis also conclusively proved that inhibition of glucanase inhibitor protein is mainly caused by recognition of an arginine as well as an isoleucine residue during the interaction of the two proteins.
Not Available
 
Date 2021-08-10T04:42:29Z
2021-08-10T04:42:29Z
2016-10-15
 
Type Research Paper
 
Identifier Johnson George K, Rosana Babu O, Vijesh Kumar I P, Eapen S J and Anandaraj M 2016. Interplay of genes in plant–pathogen interactions: In planta expression and docking studies of a beta 1,3 glucanase gene from Piper colubrinum and a glucanase inhibitor gene from Phytophthora capsici. Physiology and Molecular Biology of Plants 22: 567-573. DOI: 10.1007/s12298-016-0378-7.
0971-5894
http://krishi.icar.gov.in/jspui/handle/123456789/54179
 
Language English
 
Relation Not Available;
 
Publisher Prof. H.S. Srivastava Foundation for Science and Society